Inhibition of polymorphonuclear leukocyte functions by fluorinated nitrobenzenes |
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| Authors: | J G Elferink M Deierkauf |
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| Affiliation: | Laboratory of Medical Biochemistry, Sylvius Laboratories, University of Leiden, Wassenaarseweg 72, 2333 AL Leiden The Netherlands |
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| Abstract: | The bifunctional fluorinated nitrobenzenes, 1,5-difluoro-2,4-dinitrobenzene (DFDNB) and 4,4'-difluoro-3,3'-dinitrodiphenyl sulfone (DFDNDPS), and the monofunctional 1-fluoro-2,4-dinitrobenzene (FDNB) inhibit chemotaxis, phagocytosis, exocytosis and the respiratory burst of rabbit polymorphonuclear leukocytes. Inhibition occurs in the micromolar concentration range; the bifunctional compounds are stronger inhibitory than the monofunctional one. The inhibitory effect can be counteracted by sulfhydryl compounds and not with amino-group containing compounds. The results suggest that an interaction with vulnerable sulfhydryl groups, located in a hydrophobic surrounding, is the basis of the inhibitory effect of the fluorinated nitrobenzenes. |
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| Keywords: | Polymorphonuclear leukocytes - Fluorinated nitrobenzenes - Chemotaxis - Phagocytosis - Exocytosis - Respiratory burst CB, cytochalasin B DFDNB, 1,5-difluoro-2,4-dinitrobenzene DFDNDPS, 4,4′-difluoro-3,3′-dinitrodiphenyl sulfone FDNB, 1-fluoro-2,4-dinitrobenzene FMLP, formyl-methionyl-leucyl-phenylalanine LDH, lactate dehydrogenase PMA, phorbol myristate acetate NBT, nitroblue tetrazolium PMNs, polymorphonuclear leukocytes |
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