| 凉山半细毛羊1号染色体微卫星遗传连锁图谱的构建 |
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| 引用本文： | 张明亚,吴登俊.凉山半细毛羊1号染色体微卫星遗传连锁图谱的构建[J].遗传,2005,27(4):575-578. |
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| 作者姓名： | 张明亚 吴登俊 |
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| 作者单位： | 四川农业大学动物科技学院,雅安 625014 |
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| 基金项目： | 德国联邦教育与研究部(BMBF)资助项目 |
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| 摘 要： | 实验选择绵羊1号条染色体上的9个微卫星标记，采用父系半同胞家系群体（共387个个体）构建凉山半细毛羊1号染色体遗传连锁图。建立的资源参考家系通过20个微卫星标记进行了系谱确证。试验结果表明，9个标记的等位基因数变化范围为5~15个，杂合度在0.202~0.831之间，平均杂合度为0.617，各标记的平均多态信息含量PIC=0.604。构建的凉山半细毛羊1号条染色体遗传连锁图总长度311.0 cM，与美国肉畜中心（USDA）和国际绵羊作图中心（IMF）构建的绵羊1号条染色遗传连锁图结果基本一致。可用于下一步的QTL定位研究。
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| 关 键 词： | 微卫星标记 凉山半细毛羊 连锁图 |
| 文章编号： | 0253-9772(2005)04-0575-04 |
| 收稿时间： | 2004-05-14 |
| 修稿时间： | 2004年5月14日 |
A Linkage Map of Mirosatellite on Chromosome 1 in Liangshan Semi-wool Sheep |
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| ZHANG Ming-Ya,WU Deng-jun.A Linkage Map of Mirosatellite on Chromosome 1 in Liangshan Semi-wool Sheep[J].Hereditas,2005,27(4):575-578. |
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| Authors: | ZHANG Ming-Ya WU Deng-jun |
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| Institution: | College of Animal Sci-Tech., Sichuan Agricultural University, Ya’an 625014, China |
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| Abstract: | The 9 microsatellite markers on Chromosomes 1 were used to construct a linkage map of Liangshan semi-wool sheep with a 387 members half-sib pedigrees after paternity testing. The results were as follows: the number of alleles for 9 markers was from 5 to 15; the heterozygosity was from 0.202 to 0.831, the average heterozygosity was 0.617 and the polymorphic information content (PIC) was 0.604. Length of the linkage map of chromosome 1 we built was 311.0 cM which is in consistent with the sheep linkage map of USDA and IMF, and can be used in Quantitative Trait Loci searching in Liangshan semi-wool sheep. |
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| Keywords: | Liangshan semi-wool sheep linkage map microsatellite markers |
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