On the interaction of 2,5-dibromo-3-methyl-6-isopropylbenzoquinone (DBMIB) with bound electron carriers in spinach chloroplasts |
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| Authors: | R K Chain R Malkin |
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| Institution: | Department of Cell Physiology, University of California, Berkeley, California 94720 U.S.A. |
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| Abstract: | 2,5-Dibromo-3-methyl-6-isopropylbenzoquinone (DBMIB), when added to chloroplasts as the sole electron donor, is an effective reducing agent. Low concentrations of 2,5-dibromo-3-methyl-6-isopropylbenzoquinone reduce cytochrome f, plastocyanin, and P700 in the dark but do not reduce the high-potential form of cytochrome b559. 2,5-Dibromo-3-methyl-6-isopropylbenzoquinone appears to interact at or near the site of function of the “Rieske” iron-sulfur center, as evidenced by a shift in the g value of the electron paramagnetic resonance signal of the reduced center. |
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| Keywords: | DBMIB 2 5-Dibromo-3-methyl-6-isopropylbenzoqumone DCMU 3-(3′ 4′-dichlorophenyl)-l l-dimethylurea tetramethyl-p-hydroquinone durohydroquinone EPR electron paramagnetic resonance Tricine |
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