RNAi 在植物功能基因组中的应用

植物生物学后基因组时代的主要目标就是确定植物基因组中所有基因所具有的功能。解决这个问题的一个最直接的方法就是还原或者敲除某个在正常条件下其功能能表达出一定的可观察到的表型的特殊基因。对于这方面的研究，插入突变技术就是一个可用的工具，但是基因的随机性，致死敲除，无标记的突变体都大大地限制了这种技术的利用。RNm技术可以克服以上那些难题。它已经被广泛地应用在线虫的功能基因组上，并且所获得的有效资源还被广泛地用于植物功能基因组的分析。

Application of RNAi to Plant Functional Genome

A major challenge in the post - genome era of plant biology is to determine the functions of all the genes in the plant genome. A straightforward approach to this problem is to reduce or knock out some phenotypic special gene which can be expressed and observed under the normal conditions. Insertion mutation can be applied to this research, but it is limited by gene randomness, lethal knock- out, non tagged mutants. RNAi can greatly overcome those problems, which has been widely used in Caenorhabdifis elegans functional, genome, and the obtained effective resources have been also applied to the analysis of plant functiunal genome.