Further studies on the characterization of repetitive Rhynchosciara DNA |
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| Authors: | J Balsamo J M Hierro F J Lara |
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| Affiliation: | 1. Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China;2. School of Public Health, Anhui Medical University, Hefei 230032, Anhui, China;3. The Affiliated Zhongda Hospital, Medical School of Southeast University, Nanjing 210009, Jiangsu, China;1. Instituto de Medicina legal y Ciencias Forenses de La Rioja, Logroño, La Rioja, Spain;2. Instituto de Medicina legal y Ciencias Forenses de Badajoz, Badajoz, Spain;3. Instituto de Medicina legal y Ciencias Forenses de Burgos, Ávila, Segovia y Soria, Burgos, Spain;4. Instituto de Medicina legal y Ciencias Forenses de Cáceres, Cáceres, Spain |
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| Abstract: | Comparison between the renaturation kinetics of DNA obtained from mitotic nuclei (embryos) and polytene nuclei (salivary glands) has shown that only about one third of the most repetitive sequences are normally replicated during the polytenization process, in Rhynchosciara angelae salivary glands. The same evidence was obtained by hybridization experiments, using RNA complementary to the most repetitive DNA sequences and DNA extracted from mitotic and polytene nuclei. Hybridization results obtained with DNA fractionated in CsCl gradient and melting temperature profiles of the isolated repetitive fractions have shown that the most repetitive DNA sequences have a G—C content within the 17–27% range. |
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