| 豆豉纤溶酶高产菌株的筛选研究 |
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| 引用本文： | 张自强,张云开,陈桂光,梁智群.豆豉纤溶酶高产菌株的筛选研究[J].工业微生物,2007,37(1):67-70. |
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| 作者姓名： | 张自强 张云开 陈桂光 梁智群 |
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| 作者单位： | 广西大学生命科学与技术学院食品发酵工程研究所,南宁,530004;广西大学生命科学与技术学院食品发酵工程研究所,南宁,530004;广西大学生命科学与技术学院食品发酵工程研究所,南宁,530004;广西大学生命科学与技术学院食品发酵工程研究所,南宁,530004 |
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| 摘 要： | 以中国豆豉为材料,取6个不同来源的样品经富集培养后,用自制血纤维蛋白平板进行初筛;利用LB纤维蛋白平板复筛与血琼脂平板进行体外溶血试验相结合的方法,筛选出安全性良好并有一定纤溶活性的菌株。再对复筛得到的菌株进行摇瓶培养,用纤维蛋白平板法测定并比较不同菌株发酵液的纤溶活性。结果成功地筛选出5株纤溶活性高和通过溶血性实验的芽孢杆菌菌株,其中菌株DC-C4粗酶液的酶活稍高,达到280IU/mL。采用16S-23S rDNA序列分析法及传统的生理生化特征鉴定法对该菌株进行鉴定,确定菌株DC-C4为蜡状芽孢杆菌(Bacillus cereus)。本实验为开发新型溶栓药物及保健食品提供了实验参考依据。
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| 关 键 词： | 纤溶酶 豆豉 筛选 纤维蛋白平板 16S-23S rDNA |
Screening of fibrinolytic enzyme-producing strain DC-C4 from Chinese douchi |
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| ZHANG Zi-qiang,ZHANG Yun-kai,CHEN Gui-guang,LIANG Zhi-qun.Screening of fibrinolytic enzyme-producing strain DC-C4 from Chinese douchi[J].Industrial Microbiology,2007,37(1):67-70. |
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| Authors: | ZHANG Zi-qiang ZHANG Yun-kai CHEN Gui-guang LIANG Zhi-qun |
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| Abstract: | Five strains with high fibrinolysis activity were screened and isolated from traditional Chinese food douchi by the improved fibrin plate.The fibrinolysis activity of the fermented fluid was determined and analyzed by the thrombolysis experiment in vitro.The strain DC-C2 and DC-C4 showed the higher fibrinolysis activities which were about 280IU/mL.Comparative 16S-23S rDNA sequence analyses revealed that strain DC-C4 was very similar to Bacillus cereus.The research provided experimental evidence for developing new thrombolytic medicine and fibrinolytic functional food. |
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| Keywords: | fibrinolytic enzyme Chinese douchi screening fibrin plate 16S-23S rDNA |
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