Bacillus subtilis alpha-phosphoglucomutase is required for normal cell morphology and biofilm formation |
| |
Authors: | Lazarevic Vladimir Soldo Blazenka Médico Noël Pooley Harold Bron Sierd Karamata Dimitri |
| |
Institution: | Département de Microbiologie Fondamentale, Batiment de Biologie, Faculté de Biologie et de Médecine, Université de Lausanne, CH-1015 Lausanne, Switzerland. vladimir.lazarevic@imf.unil.ch |
| |
Abstract: | Mutations designated gtaC and gtaE that affect alpha-phosphoglucomutase activity required for interconversion of glucose 6-phosphate and alpha-glucose 1-phosphate were mapped to the Bacillus subtilis pgcA (yhxB) gene. Backcrossing of the two mutations into the 168 reference strain was accompanied by impaired alpha-phosphoglucomutase activity in the soluble cell extract fraction, altered colony and cell morphology, and resistance to phages phi29 and rho11. Altered cell morphology, reversible by additional magnesium ions, may be correlated with a deficiency in the membrane glycolipid. The deficiency in biofilm formation in gtaC and gtaE mutants may be attributed to an inability to synthesize UDP-glucose, an important intermediate in a number of cell envelope biosynthetic processes. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|