| 植物生长调节剂对转基因鱼腥藻7120生长与外源基因表达的影响 |
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| 引用本文： | 刘志伟,张晨,侯雨文,郭勇. 植物生长调节剂对转基因鱼腥藻7120生长与外源基因表达的影响[J]. 植物生理学通讯, 2004, 40(5): 567-569 |
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| 作者姓名： | 刘志伟 张晨 侯雨文 郭勇 |
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| 作者单位： | 1. 嘉应学院生物系,梅州,514015
2. 华南理工大学食品与生物工程学院,广州,510640 |
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| 摘 要： | 适宜浓度的IBA、6-BA、赤霉酸和氯化胆碱均促进转基因鱼腥藻7120生长,提高其生物量,不影响外源基因表达,但从总体上讲高浓度植物生长调节剂则抑制藻细胞生长,降低外源基因表达水平.这些植物生长调节剂混合施用促进转基因鱼腥藻生长效果不如单一因子好,外源基因表达水平也略有下降.
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| 关 键 词： | 植物生长调节剂 转基因鱼腥藻7120 生长 外源基因表达 |
| 修稿时间： | 2004-01-30 |
Effect of Plant Growth Substances on Growth and Exogenous Gene Expression of Recombinant Anabaena sp.PCC7120 |
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| LIU Zhi-Wei,,ZHANG Chen,HOU Yu-Wen,GUO Yong. Effect of Plant Growth Substances on Growth and Exogenous Gene Expression of Recombinant Anabaena sp.PCC7120[J]. Plant Physiology Communications, 2004, 40(5): 567-569 |
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| Authors: | LIU Zhi-Wei ZHANG Chen HOU Yu-Wen GUO Yong |
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| Affiliation: | LIU Zhi-Wei1,*,ZHANG Chen1,HOU Yu-Wen1,GUO Yong21Department of Biology,Jiaying College,Meizhou 514015, 2College of Food Engineering and Biotechnology,South China Univer-sity of Technology,Guangzhou 510640 |
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| Abstract: | Appropriate concentration of indole-3-butyeic acid (IBA), 6-benzyladenine (6-BA), gibberelic acid(GA3) and choline chloride (CC) could promote cell growth of recombinant Anabaena sp. PCC7120, and hadno effect on exogenous gene expression. The high concentration of IBA, 6-BA, GA3 and CC would restrain cellgrowth and reduce exogenous gene expression. When IBA, 6-BA, GA3 and CC were added to medium together,the promotion to cell growth was weaker than the action of anyone plant growth substances, and exogenousgene expression level was reduced slightly. |
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| Keywords: | plant growth substances recombinant Anabaena sp.PCC7120 growth exogenous gene expres-sion |
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