| p27kip1蛋白在胶质瘤细胞中的表达及定位研究 |
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| 引用本文： | 章倩倩,周惠,屈良鹄,王丽京.p27kip1蛋白在胶质瘤细胞中的表达及定位研究[J].生物磁学,2013(24):4627-4629,4633. |
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| 作者姓名： | 章倩倩 周惠 屈良鹄 王丽京 |
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| 作者单位： | [1]广东药学院血管生物学研究所广东广州510006 [2]中山大学生物工程中心广东广州510275 |
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| 基金项目： | 国家自然科学基金项目（31200896）;广东省医学科研基金项目（B2012181）;广东省自然科学基金项目（S2012040007658） |
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| 摘 要： | 目的：探讨胶质瘤细胞中p27酬蛋白的表达、定位，为进一步研究p27kip1在胶质瘤发生、发展过程中的功能奠定理论基础。方法：用免疫荧光方法检测U87、LN308细胞p27kiP1蛋白的定位情况；进一步分离两种细胞的细胞质与细胞核，在显微镜下观察细胞核形态并用DAPI染色分析细胞核完整性，提取蛋白用Westernblotting。方法检测分离的细胞质与细胞核蛋白的纯度，并检测p27kip1，在细胞中的表达情况。结果：成功分离了细胞的细胞浆与细胞核，并得到纯度较好的细胞浆蛋白与细胞核蛋白。确定了p27kip1蛋白主要表达于U87和LN308细胞的细胞质中。结论：p27kip1蛋白在恶性胶质瘤中可能主要表达在细胞质中，并且其亚细胞定位可能与胶质瘤的恶性程度相关。
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| 关 键 词： | p27kip1 胶质瘤 表达 定位 |
Study on the Expression and Localization of 1927kip1 in Glioma Cells |
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| ZHANG Qian-qian,ZHOU HuY,QU Liang-hu,WANG Li-jing.Study on the Expression and Localization of 1927kip1 in Glioma Cells[J].Biomagnetism,2013(24):4627-4629,4633. |
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| Authors: | ZHANG Qian-qian ZHOU HuY QU Liang-hu WANG Li-jing |
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| Institution: | 1 Vascular Biology Research Institute, Guangdong Pharmaceutical Universi(y, Guangzhou 510006, China; 2 Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Sun Yat-Sen University, Guangzhou, 510275, China) |
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| Abstract: | Objective： To investigate the expression and localization of p27kiP1; in glioma cells, and give the basis function for analysis of p27kiP1; in the tumorigenesis and development of glioma. Methods： The localization of p27kiP1; in U87 cells and LN308 cells was detected by immunofluorescence. The cytoplasm and nucleus was seperated, and the nucleus were stain with DAPI. Then the integrity of nucleu was detected under a microscope. The purity of the protein and the expression of p27kiP1; in the cytoplasm and nucleus were detected by using western blotting assay. Results： The cytoplasm and nucleus were seperated, and it was found that the p27kiP1 protein expressed mainly in the cytoplasm of U87 cells and LN308 ceUs. Conclusion： The p27kiP1 protein expressed mainly in the cytoplasm of glioma, and the subcellular localization of p27kiP1 may be consistent with their biological ftmction. |
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| Keywords: | p27kiP1 Glioma Expression Localization |
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