Development and Efficacy of Real-Time PCR in the Diagnosis of Vivax Malaria Using Field Samples in the Republic of Korea |
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| Authors: | Jung-Yeon Kim Youn-Kyoung Goo So-Young Ji Hyun-Il Shin Eun-Taek Han Yeonchul Hong Dong-Il Chung Shin-Hyung Cho Won-Ja Lee |
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| Institution: | 1. Division of Malaria and Parasitic Diseases, National Institute of Health, Korea CDC, Osong Saeng-myeong 2 ro, Osong Health Technology Administration Complex 187, Osong, Republic of Korea.; 2. Department of Parasitology and Tropical Medicine, Kyungpook National University School of Medicine, Daegu, Republic of Korea.; 3. Department of Parasitology, Kangwon National University College of Medicine, Chuncheon, Republic of Korea.; Ehime University, Japan, |
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| Abstract: | The development of sensitive, rapid, and accurate diagnostic methods for vivax malaria is essential for the effective control of malaria in the Republic of Korea, where vivax malaria patients usually show low parasitemia. In this study, a TaqMan-based real-time polymerase chain reaction (PCR) method was established and compared with other PCR-based assays, including nested PCR, loop-mediated isothermal amplification, and multiplex PCR, using samples from febrile patients with suspected vivax malaria. The established real-time PCR had a high sensitivity (99.6%) and specificity (100%). Therefore, this sensitive, specific, rapid, and quantitative real-time PCR method could be used for the routine diagnosis of vivax malaria in the laboratory of the Korea National Institute of Health. |
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