Production and characterization of ligninolytic enzymes ofBjerkandera adusta grown on wood meal/wheat bran culture and production of these enzymes using a rotary-solid fermenter |
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Authors: | Shinichi Yoshida Saori Yonehara Shigemi Minami Hyo-cheol Ha Kenji Iwahara Takashi Watanabe Yoichi Honda Masaaki Kuwahara |
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Affiliation: | (1) Wood Research Institute, Kyoto University, 611 Uji Kyoto, Japan;(2) Faculty of Agriculture, Kinki University, 631 Nara, Japan |
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Abstract: | Manganese peroxidase (MnP) and lignin peroxidase (LiP) were produced by growing a white-rot fungusBjerkandera adusta statically, on a wood meal/wheat bran culture in flasks. MnP and LiP reached their maximum activity after 6 and 19 days of inoculation, respectively. Both MnP and LiP are thought to be important enzymes in lignin biodegradation byB. adusta. Ion exchange chromatography showed thatB. adusta produced a single LiP and a single MnP enzyme in wood meal/wheat bran culture. These enzymes were separated and characterized. The molecular weight of MnP was 46,500 with a pl of 3.9. The molecular weight of LiP was estimated to be 47,000 with a pl of 3.5. Spectral analysis demonstrated that both enzymes are heme proteins. Production of these enzymes was also achieved using a rotarysolid culture fermenter. MnP, LiP and veratryl alcohol oxidase were produced byB. adusta in the fermenter. |
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Keywords: | Bjerkandera adusta lignin peroxidase manganese peroxidase rotary-solid type fermenter solid culture |
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