MKi67-siRNA可抑制QGY-7703细胞的生长

为了探讨MKI67在肝癌细胞发生发展中的作用，采用实时定量 PCR 方法检测人肝细胞癌 QGY 7703 细胞中MKI67 基因表达水平， 以及 MKI67在肝细胞癌组织和癌旁正常组织中的表达情况，设计并合成针对MKI67 的siRNA，利用脂质体转染法将其转入QGY-7703 细胞内，通过MTT和细胞集落形成实验观察MKI67-siRNA 对QGY-7703细胞生长活性和增殖能力的影响.实时定量PCR结果表明，MKI67在肝细胞癌组织中的表达水平明显高于癌旁正常组织（P< 0.01）. MTT和细胞集落形成实验结果显示，转染MKI67-siRNA 的QGY-7703细胞生长活性和集落形成率明显低于对照组（P< 0.01）.由此得出结论：MKI67 在肝癌细胞系QGY-7703细胞中的表达水平较高，且它在肝癌组织中的表达水平明显上调. 同时,MKI67-siRNA 可以有效抑制QGY-7703细胞的生长活性和增殖能力，提示MKI67可能与肝细胞癌的发生、发展相关.

MKi67–siRNA suppresses QGY-7703 Cell Growth

Abstract To explore the expression of MKi67 in human hepatocellular carcinoma, real-time-PCR (RT-PCR) was used to detect the mRNA expression level of MKi67 in human HCC tissues, the adjacent normal tissues, and human HCC cell line QGY-7703 cells. Then the chemical synthesized MKi67-siRNA, which was designed to target MKi67, was transfected into QGY-7703 cells. MTT assay and clone formation assay were used to detect the cell growth activity and proliferation capacity of QGY-7703 cells. The result showed that the expression of MKi67 mRNA in HCC tissues was higher than that in adjacent normal tissues (p < 0. 01). MTT and clone formation analyses demonstrated that the cell growth activity and clone formation ratio of MKi67-siRNA transfected QGY-7703 cells were markedly decreased (p < 0. 01). In conclusion, the MKi67 is up-regulated in HCC tissues and QGY-7703 cells, and can promotes the cell activity and proliferation ability in QGY-7703 cells. In a word, MKi67 may be related to the pathogenesis of HCC.