下调窖蛋白-1表达抑制小鼠肝癌H22细胞侵袭能力

窖蛋白-1在不同肿瘤中发挥作用不同. 本研究以小鼠肝癌细胞H22为研究对象 ，观察下调窖蛋白-1表达对H22细胞侵袭能力的影响，并探讨其可能的分子机制. 利用RT－PCR和Western印迹法检测了窖蛋白-1在H22及小鼠正常肝细胞IAR20中的 表达.结果显示，窖蛋白 1在H22中的表达高于其在IAR20中的表达，提示窖蛋白 -1高表达可能与H22细胞恶性表型有关. RNA干扰和凝集素印记实验结果显示，窖 蛋白-1－siRNA能够有效抑制窖蛋白－1mRNA和蛋白表达，并抑制细胞表面N－聚糖 β1,6GlcNAc分支形成. Transwell细胞迁移和侵袭实验结果显示，与未转染组和 siRNA 对照组比较，转染窖蛋白-1 siRNA的H22细胞迁移和侵袭数目明显减少. 本研究证明，下调窖蛋白－1表达可抑制H22细胞表面N 聚糖β1,6GlcNAc分支形 成，从而抑制细胞迁移和侵袭能力.

Knockdown of Caveolin-1 by siRNA Inhibits the Invasive Capability of Mouse Hepatoma H22 cell

Caveolin－1 is a major structural protein of caveolae and plays important roles in signal transduction and tumorigenesis. Caveolin－1 gene was regarded as both a tumor suppressor gene and an oncogene. Our previous study demonstrated that caveolin－1 was highly expressed in mouse hepatoma cell lines with lymphatic metastasis potential. However, the role of caveolin－1 in cellular migration and invasion remains unknown. Here, we used RNA interference to study the effects of silencing caveolin－1 expression on the invasive ability of mouse hepatoma H22 cells. Via targeting the mRNA region of caveolin－1, siRNA effectively suppressed caveolin－1 mRNA and protein levels. This resulted in the decreased invasive ability of H22 cells in vitro. In addition, down regulation of caveolin－1 expression suppressed the formation of N－glycan β1,6 GlcNAc branching on H22 cell surface. These results indicate that caveolin－1 could play an active role in mediating the migration and invasion of mouse hepatoma cells and might be a potential target for gene and antitumor drug therapy.