Sulfated glycosaminoglycans of cells grown in culture: Dermatan sulfate disappearance in successive fibroblast subcultures |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China;3. School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China;1. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China;2. The Science Center for Future Foods, Jiangnan University, Wuxi 214122, China;3. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China;1. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;2. The Science Center for Future Foods, Jiangnan University, Wuxi 214122, China;3. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China |
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| Abstract: | Sulfated glycosaminoglycan content of primary cultures of guinea pig embryo fibroblast in reported. It is shown that changes of sulfated glycosaminoglycans occur in successive subcultures: 1) disappearance of dermatan sulfate and 2) a slight increase in the proportion of heparitin sulfate. The sulfated glycosaminoglycan composition of the mammalian cell established lines: VERO, SIRK, Hep-2, MK-2, BGM, IB-RS-11 (S6) and 3T6 is also reported. It is shown that chondroitin sulfate AC and/or heparitin sulfate are the main glycosaminoglycans of the established cell lines (except 3T6). A possible role of these glycosaminoglycans in cell biology is discussed. |
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