Post-meiotic gene activity in spermatogenesis of the mouse |
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| Affiliation: | 1. CNRS UMR 5309, Inserm U1209, Université Grenoble Alpes, Institute for Advanced Biosciences, Grenoble 38700, France;2. Laboratory of Structural Biology, Graduate School of Advanced Science and Engineering, Research Institute for Science and Engineering, Institute for Medical-oriented Structural Biology, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan;3. Université Grenoble Alpes, Inserm U1038, CEA, BIG-BGE, Grenoble 38000, France;4. Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette 91198, France;5. TGML, platform IbiSA, Aix Marseille Univ, Inserm U1090, TAGC, Marseille 13288, France;6. Laboratoire d’Etude du Métabolisme des Médicaments, DSV/iBiTec-S/SPI, CEA Saclay, Gif-sur-Yvette 91191 Cedex, France;1. Creative Research Initiatives Center for Epigenetic Code and Diseases, Seoul National University, Seoul 08826, South Korea;2. Department of Biological Sciences, Seoul National University, Seoul 08826, South Korea;3. Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan;4. Department of Biochemistry and Molecular Biology, Korea University College of Medicine, Seoul 02841, South Korea;5. Department of Biological Sciences, Korea Advanced Institute of Science & Technology, Daejeon 34141, South Korea;6. Center for RNA Research, Institute for Basic Science, Seoul 08826, South Korea;7. Graduate School of Cancer Science and Policy, Research Institute, National Cancer Center, Goyang 10408, South Korea;8. Laboratory of Pathology and Development, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo 113-0032, Japan;9. The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan |
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| Abstract: | Mouse male germ cells at middle-late pachytene and early spermatid stages obtained by velocity sedimentation at unit gravity in albumin gradients were labelled in culture with [3H]uridine. The newly synthesized RNAs extracted from polysomes of the 2 cell types were studied by sucrose gradient fractionation and poly(U) Sepharose chromatography.The results showed that round spermatids, as well as pachytene spermatocytes, synthesize both ribosomal and polyadenylated RNA molecules. Since these latter are engaged in polysomes they are presumably active messenger RNA molecules. |
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