Use of the spot,plate and suspension test systems for the detection of the mutagenicity of environmental agents and chemical carcinogens in Neurospora crassa |
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| Affiliation: | 1. Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK;2. Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK;1. School of Economics, Hainan University, Haikou, Hainan, 570228, China;2. School of International Relation &Public Affairs, Fudan University, Shanghai, 200433, China;3. School of Liberal Arts and Sciences, North China Institute of Aerospace Engineering, Langfang, Hebei, 065000, China;1. College of Plant Protection, Laboratory of Plant Immunity, Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;2. Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China;3. School of Pharmaceutical Sciences, Sun Yat-Sen University, University Town, Guangzhou, Guangdong 510006, China;4. Tung Biomedical Sciences Centre, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China;5. Shenzhen Research Institute, City University of Hong Kong, Shenzhen, Guangdong 518057, China |
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| Abstract: | N23 and N24, selected from hundreds of ad-3 mutants, have been used as testers for the spot, plate and suspension tests in Neurospora crassa. These two testers are highly sensitive to mutagens and are revertible by a specific group of chemicals. N23 can be reverted from an adenine-dependence to adenine-independence by agents which cause base-pair substitutions whereas N24 can be reverted by frameshift mutagens. Studies described here show that spot, plate and suspension tests using testers N23 and N24 are satisfactory substitutes for the ad-3 forward-mutation system for screening the mutagenic activity of environmental agents and chemical carcinogens in N. crassa. |
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