The purification and characterization of alpha interferons and related cytokine receptors--a personal account |
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Authors: | Rubinstein Menachem |
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Institution: | aDepartment of Molecular Genetics, The Weizmann Institute of Science, POB 26, Rehovot 76100, Israel |
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Abstract: | In 1976–1977, I adapted reversed-phase HPLC (RP-HPLC) to peptide and protein purification, starting with pituitary proteins and continuing with the first successful purification to homogeneity of human leukocyte interferon (IFN- ). Using this technology, I isolated and characterized 6–8 different leukocyte interferon subtypes, which were later identified as products of the IFN- gene family. Since then, RP-HPLC became a standard procedure for isolation and analysis of proteins. The successful purification of IFN- led to the development of Roferon-A?, a drug used for the treatment of hairy cell leukemia, hepatitis C and a variety of other diseases. Later studies with my colleagues in Israel and abroad led to isolation and discovery of several cytokine receptors and binding proteins, including those of Type I IFNs, TNF and IL-18. The use of HPLC was indispensable in most of these studies. |
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Keywords: | RP-HPLC IFN TNF IFNAR2 IL-18BP sLDLR |
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