| 蛭弧菌BDG-9质粒不稳定性研究 |
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| 引用本文： | 焦鹏,马成新,王书锦.蛭弧菌BDG-9质粒不稳定性研究[J].微生物学报,2000,40(2):174-179. |
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| 作者姓名： | 焦鹏 马成新 王书锦 |
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| 作者单位： | [1]清华大学化工系生物化工研究所,北京 [2]中国科学院沈阳应用生态研究所,沈阳 |
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| 基金项目： | 国家“九·五”科技攻关项目(96-C02-02-02) |
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| 摘 要： | 本文建立了研究难于单独连续培养的专性蛭孤菌质粒稳定性的方法体系。应用此方法体系发现并证明了在传蛭孤菌ＢＤＧ－９中所含的质粒ｐＳＴⅠ存在着独特的质粒缺失现象，当蛭弧菌ＢＤＧ－９单独培养时，在通常决定质粒稳定性的复制功能和分配功能都正常的情况之下，随着细胞的传代，质粒ｐＳＴⅠ逐渐丢失，蛭弧菌的生长繁殖也逐渐停止，表明质粒ｐＳＴⅠ的存在对ＢＤＧ－９细胞单独生长有重要作用。
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| 关 键 词： | 蛭弧菌 复制功能 分配功能 质粒稳定性 质粒丢失 |
| 文章编号： | 0001-6209(2000)02-0174-79 |
| 修稿时间： | 1999-01-25 |
STUDY ON THE PLASMID INSTABILITY OF BDELLOVIBRIO BDG-9 |
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| Jiao Peng
Ma Chengxin
,Wang Shujin.STUDY ON THE PLASMID INSTABILITY OF BDELLOVIBRIO BDG-9[J].Acta Microbiologica Sinica,2000,40(2):174-179. |
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| Authors: | Jiao Peng
Ma Chengxin Wang Shujin |
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| Institution: | Jiao Peng
;(Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 100084);Ma Chengxin
,Wang Shujin
;(Shengya ng Applied Ecology Institute, Sinica of China, 110015) |
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| Abstract: | A new method system was established in this paper to study the plasmid in stability of Bdellovibrio BDG-9. Using this system, it was found that when BDG-9 was cultured singly on the SMB plate, plasmid pST I was unstable although pST I still replicated and distributed to progeny cell normally. The results showed that the pST I copy number in single cell of BDG-9 decreased gradually to zero with the propagation of BDG-9. Additionally, plasmid pST I was very important for the growth of BDG-9, and with the lacking of pST I s, the growth and propagation of BDG-9 ceased. |
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| Keywords: | Bdellovibrio Palsmid instability Replication function Distribution function |
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