Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein |
| |
Authors: | Carolina Saravia Paola Martinez Carolina Lopez Claudia Reyes |
| |
Institution: | a Fundación Instituto de Inmunología de Colombia, Molecular Biology, Carrera 50#26-20, Bogota, Colombia b Universidad de Los Andes, Bogota, Colombia c Universidad Nacional de Colombia, Bogota, Colombia d Universidad del Rosario, Bogota, Colombia |
| |
Abstract: | Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide’s ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria. |
| |
Keywords: | Malaria Plasmodium vivax Duffy binding protein (DBP) Universal epitopes |
本文献已被 ScienceDirect 等数据库收录! |
|