The gene family encoding the Arabidopsis thaliana translation elongation factor EF-1 alpha: molecular cloning, characterization and expression |
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Authors: | Michè le Axelos, Claude Bardet, Thierry Liboz, Agathe Le Van Thai, Catherine Curie Bernard Lescure |
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Affiliation: | (1) Laboratoire des Relations Plantes-Microorganismes, CNRS-INRA, BP 27, F-31326 Castanet-Tolosan, France;(2) Centre de Physiologic Végétale, Université Paul Sabatier, 118 route de Narbonne, F-31062 Toulouse, France |
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Abstract: | Summary The gene family encoding the Arabidopsis thaliana translation elongation factor (EF-1 ) was analysed. This family contains four genes (A1-A4) organized in a similar manner in different varieties of Arabidopsis. Based upon both their physical separation and a comparison of their sequences, it is suggested that the A4 gene and the A1, A2, and A3 genes constitute two distinct subfamilies within the genome. By introducing chimaeric gene constructs into Arabidopsis cells, we showed that the Al gene promoter mediates a transient expression about twofold higher than that obtained using the CaMV 35 S promoter. This expression depends on a 348 by DNA fragment extending from –982 to –634 by upstream of the initiation codon. This element contains a characteristic telomeric sequence (AACCCTAA) which is also found in the promoters of the A2 and A4 genes as well as in the promoters of the Drosophila EF-1 F1 gene and of several highly expressed plant genes. |
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Keywords: | Translation elongation factor genes Arabidopsis thaliana Promoter analysis Telomere |
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