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Secretion of the antibacterial recombinant protein enbocin
Authors:Goo Tae Won  Yun Eun Young  Kim Sung Wan  Choi Kwang Ho  Kang Seok Woo  Kwon Kisang  Choi Joung-Soon  Kwon O-Yu
Affiliation:Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA, Suwon 441-100, Korea.
Abstract:The insect baculovirus expression vector system (BEVS) is useful for the production of biologically active recombinant proteins. However, the overexpression of foreign proteins in this system often results in misfolded proteins and the formation of protein aggregates. To overcome this limitation, we have developed a versatile baculovirus expression and secretion system using the Bombyx mori protein disulfide isomerase (bPDI) as a fusion partner. bPDI gene fusion improved the secretion and antibacterial activity of recombinant enbocin proteins. Thus, bPDI gene fusion is a useful addition to the BEVS for the large-scale production of bioactive recombinant proteins.
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