An in vitro comparison of a new vinyl chalcogenide and sodium selenate on adenosine deaminase activity of human leukocytes |
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Authors: | Bellé Luziane Potrich Bitencourt Paula Eliete Rodrigues Abdalla Faida Husein Guerra Robson Brum Funchal Claudia Moretto Maria Beatriz |
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Institution: | aPharmacology Graduate Program, Health Sciences Center, Federal University of Santa Maria, 97105-900 – Santa Maria, RS, Brazil;bFederal institute of Education, Science and Technology of Rio Grande do Sul, Sertão, RS, Brazil;cMethodist University Center, Porto Alegre, RS, Brazil |
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Abstract: | Selenium (Se) is a dietary essential trace element with important biological roles. Sodium selenate (Na2SeO4) is an inorganic Se compound used in human and animal nutrition that acts as precursor for selenoprotein synthesis. The organoselenium 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one (C21H2HOSe) is an α,β-unsaturated ketone functionalized vinyl chalcogenide that has been found as a potential tool in organic synthesis. Adenosine deaminase (ADA) is an important enzyme in the degradation of adenine nucleotides. In this study, we investigated the in vitro effects of both Se compounds on ADA activity and cell viability in leukocyte suspension (LS) of healthy donors (n = 12). We first observed an inhibition of ADA activity using 0.1 μM of 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one, and an increase in cellular viability when 30 μM were used. However, we did not observe alterations in the presence of sodium selenate. Moreover, both Se compounds did not alter lactate dehydrogenase activity and thiobarbituric acid reactive substance levels. These results suggest that the inhibition of ADA activity caused by α,β-unsaturated ketone may affect the adenosine levels in LS and modulate cell viability, attenuating conditions that involve the activation of the immune system. |
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Keywords: | Cell viability Adenosine deaminase activity Leukocytes suspension Selenium compounds |
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