Mutation to rifampicin resistance at the beginning of the RNA polymerase beta subunit gene in Escherichia coli期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Mutation to rifampicin resistance at the beginning of the RNA polymerase beta subunit gene in Escherichia coli

Authors:	N A Lisitsyn E D Sverdlov E P Moiseyeva O N Danilevskaya and V G Nikiforov

Institution:	(1) Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Ul. Vavilova 32, 117988 Moscow V-334, USSR;(2) Institute of Molecular Genetics, USSR Academy of Sciences, PJ. Kourchatova 46, 123182 Moscow, USSR

Abstract:	Summary The unusual recombinant plasmid pRC19 carrying the N-terminal fragment of the Escherichia coli RNA polymerase rpoB gene was found to specify high level rifampicin resistance of E. coli cells. Sequence analysis of this plasmid revealed one substitution only: transversion GT, leading to amino acid substitution Val¹⁴⁶Phe. This mutational change marks the second domain of the subunit involved in rifampicin binding.

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