SNP和cPTIO对NaCl胁迫下拟南芥的生理影响

以拟南芥为材料,研究了0.5mmol/L的外源一氧化氮供体硝普纳(SNP)和200mmol/L的一氧化氮专一清除剂(cPTIO)对200mmol/LNaCl胁迫下对拟南芥的生理影响。0.5mmol/LSNP和200mmol/LcPTIO预处理2h后,加入200mmol/LNaCl。不同处理的拟南芥加入200mmol/LNaCl后,每隔12h取样一次,观察叶片生长情况、测定过氧化物酶(POD)、超氧化物歧化酶(SOD)、丙二醛(MDA)和超氧阴离子(O■)的变化。结果表明0.5mmol/L的SNP能缓解200mmol/LNaCl胁迫伤害,促进盐胁迫下幼苗的生长,显著提高抗氧化酶系统中POD和SOD活性,显著降低MDA和O■的含量,从而提高植株的抗盐性,而NO专一清除剂cPTIO能逆转SNP的上述效应。

Effects of SNP and cPTIO on physiology of Arabidopsis seedling under NaCl stress

Three groups of 8-week-old Arabidopsis seedlings were cultured,they were respectively pretreated with water,0.5 mmol/L SNP and 200 mmol/L cPTIO for 2 h,after that,200 mmol/L NaCl was added. Fresh NaCl solution was applied every day and some physiological indices such as peroxidase(POD),superoxide dismutase(SOD),malondialdehyde(MDA)and superoxide anion(O■)were determined 12 h later. The results showed that SNP could relieve NaCl stress injuries,and promote the growth of seedlings under salt stress,significantly improve the antioxidant enzyme system,POD and SOD activity,significantly reduce MDA and O■ content,thereby enhancing the salt tolerance of plants,while the NO specific scavenger cPTIO could reverse the above effects of SNP.