Immobilized lipase on macroporous polystyrene modified by PAMAM-dendrimer and their enzymatic hydrolysis |
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| Institution: | 1. State Key Laboratory of Applied Organic Chemistry, Institute of Biochemical Engineering & Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;2. The First Affiliated Hospital of Lanzhou University, Lanzhou University, Lanzhou 730000, China;1. Ryazan State Medical University named after academician IP Pavlov, Visokovoltnaya St. 9, Ryazan 390026, Russia;2. RUDN University, Miklukho–Maklai St. 6, Moscow 117198, Russia;3. Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Baltiyskaya St. 8, Moscow 125315, Russia;4. Department of Biochemistry, Orenburg State Medical University, Sovetskaya St. 6, Orenburg 460000, Russia;5. Department of Biochemistry, South Ural State Medical University, Vorovskogo St. 64, Chelyabinsk, 454048, Russia;6. Laboratory of Biotechnology and Applied Bioelementology, Yaroslavl State University, Sovetskaya St. 14, Yaroslavl, 150000, Russia;7. All-Russian Research Institute of Medicinal and Aromatic Plants (VILAR), Grina St. 7, Moscow, 117216, Russia;8. Institute of Bioelementology (Russian Satellite Centre of Trace Element—Institute for UNESCO), Orenburg State University, Pobedy Ave. 13, Orenburg 460352, Russia;1. Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University (BUAA), Beijing 100191, China;2. State Key Laboratories of Transducer Technology, National Engineering Lab for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;1. College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;2. Baling Company, China Petroleum & Chemical Corporation, China |
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| Abstract: | The novel enzyme carrier, polyamidoamine (PAMAM) dendrimers modified macroporous polystyrene, has been synthesized by Michael addition and firstly used in the immobilization of porcine pancreas lipase (PPL) effectively by covalent attachment. The resulting carrier was characterized with the Fourier transform infrared spectra (FT-IR), scanning electron microscopy (SEM), elemental analysis and thermogravimetric (TG) analysis. Meanwhile, the amount of immobilized lipase was up to 100 mg g−1 support and the factors related with the enzyme activity were investigated. The immobilization of the PPL improved their performance in wider ranges of pH and temperature. Thermal stability of the immobilized lipase also increased dramatically in comparison with the free ones and the immobilized lipase exhibited a favorable denaturant tolerance. As a biocatalyst, the immobilized lipase for batch hydrolysis of olive oil emulsion retained 85% activity after 10 times of recycling. This well-reusability of immobilized lipase was very valuable and meaningful in enzyme technology. |
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| Keywords: | Macroporous polystyrene PAMAM dendrimers Immobilized enzymes Lipase Enzyme technology Biocatalysis |
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