A novel and efficient method for the isolation and purification of polysaccharides from lily bulbs by Saccharomyces cerevisiae fermentation |
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| Institution: | 1. School of Biotechnology and Food Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China;2. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, PR China;1. Department of Biochemical Sciences, University of Roma La Sapienza, 00161 Rome, Italy;2. Department of Experimental Medicine, University of Roma La Sapienza, 00161 Rome, Italy;3. Department of Pediatrics, University of Roma La Sapienza, 00161 Rome, Italy;1. Department of Urology and Kidney Transplantation Center, University “Martin-Luther” Halle, Halle/Saale, Germany;2. Uroclinic, Robotic Surgery Center, Sophiahemmet Hospital, Stockholm, Sweden;3. Dipartimento Minimally Invasive Urologic Surgery, Romolo Hospital, Rocca di Neto (KR), Italy;1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China;2. National Engineering Research Center for Functional Food, Jiangnan University, Wuxi 214122, PR China;3. GuoLi Dendrobium nobile Company Limited, ChiShui 564700, PR China;3. Institute of Pharmacology, Center for Cardiovascular Research, Charité-Universitaetsmedizin Berlin, 10115 Berlin, Germany;5. Department of Nuclear Medicine, University Hospital Wuerzburg, 97080 Wuerzburg, Germany;6. Department of Veterinary Pathology, College of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany;12. Department of Prevention, Rehabilitation, and Sports Medicine, Technische Universitaet Muenchen, 80809 Muenchen, Germany;8. DZHK (German Center for Cardiovascular Research), Munich Heart Alliance, 80809 Munich, Germany;9. Department of Sports Medicine, Humboldt University/Charité-Universitaetsmedizin Berlin, 10115 Berlin, Germany;10. Division of Endocrinology and Metabolism, University of Pittsburgh, Pittsburgh, Pennsylvania 15261;4. DZHK (German Center for Cardiovascular Research), 10115 Berlin, Germany |
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| Abstract: | A water-soluble polysaccharide from lily bulbs was isolated and purified by Saccharomyces cerevisiae fermentation. Proteins present in lily bulb extract were removed by extracellular proteases secreted by S. cerevisiae during fermentation. This novel method differs from traditional protein removal methods. A suitable yeast strain was selected. Culture conditions were optimized. Response surface methodology (RSM) was utilized to evaluate the effects of variables on the lily polysaccharide (LP) yield and the protein removal ratio (PRR). The results of applying RSM revealed that the optimum fermentation conditions were 87.5 g L?1 lily bulb powder, pH 5.6, and temperature 27.9 °C. When lily bulb extract was cultured with S. cerevisae under optimum conditions, the LP yield and the PRR were 6.56% and 91.46%, respectively. These values are in close agreement with the value predicted by the model. The resulting LP curding was further purified by DEAE Sepharose Fast Flow chromatography after isolation by alcohol precipitation post-fermentation. DEAE chromatography resulted in a fraction, LP-1 (yield: 4.46%) with a molecular weight of 65.0 kDa. LP-1 consisted of glucose and mannose in a molar ratio of 1:1.2. |
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| Keywords: | Lily polysaccharide Isolation Purification Characterization |
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