New at-line flow cytometric protocols for determining carotenoid content and cell viability during Rhodosporidium toruloides NCYC 921 batch growth |
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| Affiliation: | 1. Graduation Program of Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), Centro Politécnico, C.P. 19011, 81-531-980 Curitiba, PR, Brazil;2. OuroFino Agrociência, Cravinhos, SP 14140-000, Brazil;3. Federal University of Technology of Paraná (UTFPR) – Campus Toledo, Rua Cristo Rei, 19 Toledo, PR 85902-490, Brazil;1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, PR China;2. School of Pharmaceutical Science, Nanjing Tech University, Nanjing 211800, PR China;1. Department of Materials and Bioprocess Engineering, School of Chemical Engineering, University of Campinas, 13083-852, Campinas, São Paulo, Brazil;2. Center for Molecular Biology and Genetic Engineering, University of Campinas, 13083-875, Campinas, São Paulo, Brazil |
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| Abstract: | Rhodosporidium toruloides NCYC 921 batch growth was monitored as a means to evaluate the yeast biomass potential as a source for the production of carotenoids and other lipids.Carotenoid content, cell viability and size were assessed by multiparameter flow cytometry. The saponifiable lipid fraction was assayed by gas–liquid chromatography.The carotenoid production increased during the stationary phase, reaching 78 μg/g while the total fatty acid content attained 32% (w/w) at the end of the fermentation. The fatty acid profile was suitable for biodiesel purposes.As the yeast cells entered the stationary phase, the proportion of cells with depolarised mitochondrial membrane and cells with permeabilised cytoplasmic membrane increased, attaining 65% and 14%, respectively. Nevertheless, a high proportion of cells (82%) showed esterase activity.These results demonstrated that flow cytometry can be a powerful at-line technique to monitor the total carotenoids and cell viability during the yeast growth, being useful for the yeast process optimisation at lab and pilot scales. |
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| Keywords: | Carotenoids Fatty acids Flow cytometry |
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