Functional cello-oligosaccharides production from the corncob residues of xylo-oligosaccharides manufacture |
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| Institution: | 1. Institute of Feed Science, MoE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, Hangzhou 310058, China;2. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;1. State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, PR China;2. School of Bioengineering, Qilu University of Technology, Shandong Academy of Science, Jinan, 250353, PR China;3. College of Life Science, Shandong Normal University, Jinan, 250014, PR China;4. Department of Radiology, Qilu Hospital of Shandong University, Jinan, 250012, PR China;1. Centre National de la Recherche Scientifique, Laboratoire de Chimie des Polymères Organiques, UMR 5629, IPB/ENSCBP, 16 avenue Pey-Berland, F-33607 Pessac Cedex, France;2. University of Bordeaux, Laboratoire de Chimie des Polymères Organiques, UMR 5629, IPB/ENSCBP, 16 avenue Pey-Berland, F-33607 Pessac Cedex, France |
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| Abstract: | An integrated process has been developed, consisting of the “adsorption–separation” of cellulase enzymes to selectively remove β-glucosidase, and multi-stage enzymatic hydrolysis of corncob residues from xylo-oligosaccharides manufacture with the β-glucosidase deficient cellulase, aiming to obtain a high yield of cello-oligosaccharides production. After the “adsorption–separation” process, 79.50% of the endo-glucanase was retained in substrate, whereas 90.67% of β-glucosidase was removed with the separated liquid fraction, utilizing the different adsorbability of these enzymes to the substrate. A three-stage enzymatic hydrolysis of corncob residues with the β-glucosidase deficient cellulase was proposed in which the first, the second and the third stage were conducted for 6, 6 h and 12 h, respectively. Analysis indicated that the removal of hydrolysis products (glucose and cello-oligosaccharides) at each stage improved cello-oligosaccharides productivity and enzymatic hydrolysis yield. The cello-oligosaccharides yield and enzymatic hydrolysis yield in three-stage enzymatic hydrolysis were significantly improved to 51.78% and 75.56%, respectively, which were 36.00% and 25.10% higher than single-stage hydrolysis with original cellulase enzymes. |
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| Keywords: | Corncob residues Cellulase Adsorbability Enzymatic hydrolysis Cello-oligosaccharide |
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