Ultrasound assisted lipase catalysed synthesis of isoamyl butyrate |
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| Institution: | 1. Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China;2. State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin 300072, PR China;3. Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin 300072, PR China;4. The Co-Innovation Center of Chemistry and Chemical Engineering of Tianjin, Tianjin 300072, PR China;1. Chemical Engineering Department, Institute of Chemical Technology, Matunga, Mumbai 40019, India;2. Department of Oils, Oleochemicals and surfactant Technology, Institute of Chemical Technology, Matunga, Mumbai 40019, India;1. Chemical Engineering Department, Institute of Chemical Technology, Matunga, Mumbai 40019, India;2. Department of Oils, Oleochemicals and Surfactant Technology, Institute of Chemical Technology, Matunga, Mumbai 40019, India;1. Jiangsu Engineering Technology Research Center for Functional Textiles, Jiangnan University, Wuxi 214122, China;2. International Joint Research Laboratory for Textile and Fiber Bioprocesses, Jiangnan University, Wuxi 214122, China;3. Department of Chemical Engineering, The Institute of Chemical Technology, Mumbai 400019, India;4. CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal;5. Institute of Polymers and Composites (IPC) and Institute of Nanostructures, Nanomodelling and Nanofabrication (I3N), University of Minho, Campus de Azuém, Guimarães 4800-058, Portugal;6. Centre of Chemistry, Departament of Chemistry, Universidade do Minho, Campus Gualtar, Braga 4710-057, Portugal |
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| Abstract: | The present work illustrates the incorporation of ultrasound and its improved impact in the lipase catalysed esterification. Synthesis of isoamyl butyrate from isoamyl alcohol and butyric acid using immobilised Novozym 435, has been carried out in the presence of ultrasound. The optimisation of various parameters affecting the synthesis of ester in presence of ultrasound was done. The systematic experimentation involves change of one working parameter at one time while keeping the others constant. For the maximum conversion, optimum parameters such as the ultrasound of 25 kHz frequency with power of 70 W, at the temperature of 60 °C with stirring speed of 80 rpm, mole ratio of alcohol:acid followed as 2:1, use of molecular sieves weighing 2 g, with immobilised enzyme loading of 2% (m/v) and duty cycle of 83%, were obtained. The optimum parameters collectively, gave 96% conversion of the product in 3 h as compared with 10 h in absence of ultrasound. The immobilised biocatalyst, Novozym 435 has an added benefit of reusability till 7 repetitive cycles. Besides, the synthesis is executed in the solvent free system that contributes the production of flavour in greener way. |
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| Keywords: | Isoamyl butyrate Ultrasound Immobilised lipase Novozym 435 Fruity banana flavour Optimisation |
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