Constitutive mosaic aneuploidy is a unique genetic feature widespread in the Leishmania genus |
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| Institution: | 1. University Montpellier 1, Faculty of Medicine, Laboratory of Parasitology–Mycology, Montpellier, France;2. CNRS 5290, IRD 224, University Montpellier 1&2 (UMR “MiVEGEC”), Montpellier, France;3. Centre Hospitalier Universitaire (University Hospital Centre), Montpellier, France;1. Department of Medical Parasitology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia;2. Department of Microbiology, Immunology and Parasitology, School of Medicine, Addis Ababa University, Addis Ababa, Ethiopia;3. Department of Zoological Sciences, College of Natural Science, Addis Ababa University, Addis Ababa, Ethiopia;4. Department of Parasitology, Faculty of Science, Charles University in Prague, Vinicna 7, 12844, Prague 2, Czech Republic;5. Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia;6. Department of Microbiology and Molecular Genetics, The Institute for Medical Research Israel-Canada, The Kuvin Centre for the Study of Infectious and Tropical Diseases, The Hebrew University – Hadassah Medical School, Jerusalem, Israel;7. School of Veterinary Medicine, Hebrew University, P.O. Box 12, Rehovot, 76100, Jerusalem, Israel;1. Centre de Recherche en Infectiologie du Centre de Recherche du CHU de Québec, Pavillon CHUL, and Département de Microbiologie-Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Canada;2. Plate-forme Protéomique du Centre de Génomique de Québec, CHU de Québec, Pavillon CHUL, Université Laval, Québec, Canada;1. Laboratório Especial de Ciclo Celular (LECC), Center of Toxins, Immune Response and Cell Signaling (CeTICS), Butantan Institute, São Paulo, SP, Brazil;2. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil;3. Division of Biological Chemistry and Drug Discovery, School of Life Sciences, Dow Street, University of Dundee, Dundee, UK;4. The Wellcome Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK |
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| Abstract: | Using fluorescence in situ hybridization, we determined the ploidy of four species of Leishmania: Leishmania infantum, Leishmania donovani, Leishmania tropica and Leishmania amazonensis. We found that each cell in a strain possesses a combination of mono-, di- and trisomies for all chromosomes; ploidy patterns were different among all strains/species. These results extend those we previously described in Leishmania major, demonstrating that mosaic aneuploidy is a genetic feature widespread to the Leishmania genus. In addition to the genetic consequences induced by this mosaicism, the apparent absence of alternation between haploid/diploid stages questions the modality of genetic exchange in Leishmania sp. |
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| Keywords: | Genome Chromosome Aneuploidy Mosaicism Genetic exchange |
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