Characterization and antioxidant activity for exopolysaccharide from submerged culture of Boletus aereus |
| |
Institution: | 1. Nanchong Academy of Agricultural Sciences, Nongke Road, Nanchong, Sichuan Province 637000, China;2. College of environmental science and Engineering, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China;3. Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Sciences, China West Normal University, 1# Shida Road, Nanchong, Sichuan Province 637009, China |
| |
Abstract: | Three polysaccharide fractions (designated as Fr-I, Fr-II and Fr-III) were successfully purified from the crude exopolysaccharide (EPS) produced from submerged culture of Boletus aereus by gel filtration chromatography on Sepharose CL-6B. The size exclusion chromatography/multi-angle laser light scattering (SEC/MALLS) system showed that the average molecular weights (Mws) of these three fractions were 1.365 × 106, 1.048 × 105 and 2.471 × 104 g/mol, respectively. The SEC/MALLS also revealed that the molecular conformation of the Fr-I was a random coil, with Fr-II being a rigid rod in aqueous solution. Moreover, monosaccharide composition analysis indicated that Fr-I was mainly composed of glucose, while both of Fr-II and Fr-III were mainly composed of mannose and glucose. Then, FT-IR spectral analysis of the purified EPS revealed prominent characteristic groups. Furthermore, thermo gravimetric analysis (TGA) indicated the degradation temperature of the Fr-I (170 °C) was higher than those of Fr-II (156 °C) and Fr-III (155 °C). Finally, on the basis of the antioxidant activity test in vitro, Fr-I exhibited the highest antioxidant ability among these samples, which might be attributed to the monosaccharide composition and molecular weight in the EPS fraction. |
| |
Keywords: | Exopolysaccharide Thermogravimetry Antioxidant activity SEC/MALLS |
本文献已被 ScienceDirect 等数据库收录! |
|