Significance of metal ion supplementation in the fermentation medium on the structure and anti-tumor activity of Tuber polysaccharides produced by submerged culture of Tuber melanosporum |
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| Institution: | 1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China;2. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;1. Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture; Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, PR China;2. Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, Bellaterra, 08193 Barcelona, Spain;3. Biophotonic Nanosensors Laboratory, Centro de Investigaciones en Óptica, A. C., Loma del Bosque 115, Lomas del Campestre, León, Guanajuato 37150, Mexico;4. Departament d''Enginyeria Química, Universitat Autònoma de Barcelona, Bellaterra, Barcelona 08193, Spain;5. Institucio Catalana de Recerca i Estudis Avançats (ICREA), Barcelona 08010, Spain;1. Key Laboratory of Textile Science and Technology, Ministry of Education, College of Textiles, Donghua University, Shanghai 201620, China;2. Innovation Center for Textile Science and Technology, Donghua University, Shanghai 200051, China;1. Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, 87020-900, Maringá, PR, Brazil;2. Departamento de Química e Bioquímica, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, 19060-900, Presidente Prudente, SP, Brazil;3. Centro de Investigaciones Biológicas, CSIC, 28006, Madrid, Spain;4. Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, 13418-900, Piracicaba, SP, Brazil;1. Université de Technologie de Compiègne, Unité Transformations Intégrées de la Matière Renouvelable – EA 4297/ESCOM, Centre de Recherches de Royallieu, BP 20529-60205 Compiègne Cedex, France;2. Ecole supérieure de chimie organique et minérale, EA 4297 TIMR, 1 allée du réseau J.M. Buckmaster, 60200 Compiègne, France;3. Institut des Sciences de la Vie, Université catholique de Louvain, Croix du Sud 2, L7.05.08 B-1348 Louvain-la-Neuve, Belgium;4. Laboratoire d’Ingénierie des Matériaux de Bretagne – EA 4250 Université de Bretagne Sud, IUT de Lorient, Centre de Recherche, rue de Saint Maudé, BP 92116-56321 Lorient Cedex, France |
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| Abstract: | The significance of metal ion supplementation in the fermentation medium on the structure and anti-tumor activity of Tuber polysaccharides was systematically studied in the submerged fermentation of Tuber melanosporum. The lowest weight-average molecular weight (Mw) (i.e., 115.3 × 104 g/mol) of intracellular polysaccharides (IPS) was obtained when Mg2+ and K+ was added in the fermentation medium. The IPS with the lower Mw exhibited a higher inhibition ratio against S-180 tumor cells. The compact conformation of extracellular polysaccharides (EPS) was formed when only K+ was supplied in the fermentation medium. Interestingly, EPS with compact conformation exhibited a higher inhibition ratio (i.e., 59.2%) than EPS with branched polymer chain (i.e., 9.2%) against A549 tumor cells. The highest inhibition ratio for EPS with α-glycosidic linkages against the tumor cell line HepG2 reached 32.2% when Mg2+ or K+ was supplied in the fermentation medium. The addition of metal ion Mg2+, K+, and their combination to the fermentation medium is a vital factor affecting the structures of Tuber polysaccharides, which further determine their anti-tumor activities. The information obtained in this work will be useful for the efficient and directed production of polysaccharides with anti-tumor activities by the submerged fermentation of edible fungi mycelium. |
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| Keywords: | Metal ion Polysaccharides Structure Anti-tumor activity |
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