Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production |
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| Institution: | 1. Department of Biotechnology, University of Chemistry and Technology, Prague, Czech Republic;2. Department of Biotechnology and Life Sciences, University of Insubria, Varese, Italy;3. \"The Protein Factory\" Research Center, Politecnico of Milano, ICRM CNR Milano and University of Insubria, Varese, Italy;1. College of Bioscience and Engineering, Jiangxi Agricultural University, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Nanchang 330045, China;2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China;1. Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy;2. Department of Life Sciences and Technology / Bioprocess Engineering, Beuth University of Applied Sciences Berlin, Seestrasse 64, 13347 Berlin, Germany |
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| Abstract: | In this study, a new cell immobilization technique is presented. Cells of Clostridium acetobutylicum DSM 792 form a macroporous aggregate through cryogelation with concomitant crosslinking together with activated polyethyleneimine (PEI) and poly(vinyl alcohol) (PVA). The cell based cryogel presents a highly porous, elastic structure with walls consisting of densely packed crosslinked cells. The immobilization process maintained the viability of cells as new bacterial cells were observed when gel-plugs were incubated in liquid medium, glucose was consumed and solvent production was observed. Solvent production was improved 2.7-fold in immobilized cells in comparison to free cells. It was possible to reuse the gel-plugs 3–5 times in partial or completely fresh medium, reaching a maximum butanol concentration in the broth of 18.2 g/l and yield of 0.41 (g/g) in one of the cycles. The use of cells based cryogels can be a good alternative for improvement of acetone-butanol-ethanol (ABE) process as cells are immobilized in a macroporous structure with low limitations for mass transfer with potential for high yield production. |
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| Keywords: | Cell immobilization Cryogel Butanol |
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