Identification and biochemical characteristics of lipopeptides from Bacillus mojavensis A21 |
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| Institution: | 1. Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d’Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia;2. ProBioGEM – EA1026, Polytech’Lille/IUTA, Université Lille-Nord de France, F-59655 Villeneuve d’Ascq, France;1. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China;2. Oil Production Research Institute, Shengli Oil Field Ltd. Co. SinoPEC, Beijing, China;1. Food Technology Division, FIPLY, Bhabha Atomic Research Centre, Mumbai 400 085, India;2. Bio-Organic Division, Bhabha Atomic Research Centre, Mumbai 400 085, India;1. Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d’Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia;2. Laboratoire d’Histologie et d’Embryologie, Faculté deMédecine à Sfax, Route Majida Boulila, 3028 Sfax, Tunisia;3. Laboratoire de Pharmacologie, Faculté de Médecine à Sfax, Route Majida Boulila, 3028 Sfax, Tunisia;4. ProBioGEM-EA1026, Polytech’Lille/IUTA, Université Lille-Nord de France, 59655 Villeneuve d’Asq, France;1. Higher Institute of Biotechnology, Tunisia;2. Unit Enzymes and Bioconversion, National School of Engineers, Tunisia;1. Department of Natural Sciences, FCEFQyN, National University of Río Cuarto, Ruta Nacional 36 Km 601, Córdoba, Argentina;2. Department of Molecular Biology, FCEFQyN, National University of Río Cuarto, Ruta Nacional 36 Km 601, Córdoba, Argentina;3. IBBM (Instituto de Biotecnología y Biología Molecular), CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina |
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| Abstract: | This study reports the potential of a marine bacterium, Bacillus mojavensis A21, to produce lipopeptide biosurfactants. The crude lipopeptide mixture was found to be very effective in reducing surface tension to 31 mN m?1. PCR experiments using degenerate primers revealed the presence of nonribosomal peptide synthetases genes implied in the biosyntheses of fengycin and surfactin. Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) performed on whole cells of B. mojavensis A21 confirmed the presence of lipopeptides identified as members of surfactin and fengycin families. Further, a detailed analysis performed by MALDI-TOF-TOF revealed the presence of pumilacidin compounds. The crude lipopeptide mixture was tested for its inhibitory activity against Gram-positive and Gram-negative bacteria, and fungal strains. It was found to display significant antimicrobial activity. Strain A21 lipopeptide mixture was insensitive to proteolytic enzymes, stable between pH 3.0 and 11.0, and resistant to high temperature. Production of lipopeptides is a characteristic of several Bacillus species, but to our knowledge this is the first report involving identification of pumilacidin, surfactin and fengycin isoforms in a B. mojavensis strain. |
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| Keywords: | NRPS PCR MALDI-TOF-MS Fengycin Surfactin Pumilacidin |
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