Biotransformation of the SDG in defatted flaxseed into END co-cultured by three single bacterial colonies |
| |
| Affiliation: | 1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China;2. School of Basic Medical Sciences, Peking University, Beijing 100191, China;3. Genomics Research Center, Harbin Medical University, Harbin 150081, China;4. Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, AB T2N 4N1, Canada;1. Department of Chemical Engineering, Federal University of São Carlos-UFSCar, Rodovia Washington Luiz, km 235, São Carlos, SP 13565-905, Brazil;2. Institute of Chemistry, São Paulo State University-UNESP, Rua Prof. Francisco Degni s/n, Araraquara, SP 14800-900, Brazil;3. Department of Physics, Federal University of São Carlos-UFSCar, Rodovia Washington Luiz, km 235, São Carlos, SP, 13565-905, Brazil;1. Metabolomics Australia, School of Botany, The University of Melbourne, Parkville, 3010 Victoria, Australia;2. Department of Chemistry, Faculty of Sciences, Shahid Beheshti University, G.C., 1983963113 Tehran, Iran;3. Department of Plant Sciences, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran;4. Medicinal and Natural Products Chemistry Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran;5. Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019, USA;6. Australian Centre for Plant Functional Genomics, School of Botany, The University of Melbourne, Parkville, 3010 Victoria, Australia;1. Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 11, 783 71 Olomouc, Czech Republic;2. Department of Nanobiotechnology, Institute of Nanobiology and Structural Biology of GCRC, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic;1. National Health and Environmental Effects Research Laboratory, United States Environmental Protection Agency, RTP, NC, USA;2. National Exposure Research Laboratory, United States Environmental Protection Agency, Cincinnati, OH, USA;3. Division of Geographic Medicine and Infectious Diseases, Dept. of Medicine, Tufts Medical Center, Boston, MA, USA;4. EPA Region 1 (New England), United States Environmental Protection Agency, Boston, MA, USA;1. Veterinary and Biomedical Sciences, University of Minnesota, MN, USA;2. Minnesota Supercomputing Institute, University of Minnesota, MN, USA;1. Laboratoire Information Génomique & Structurale, UMR7256 (IMM FR3479) CNRS Aix-Marseille Université, 163 Avenue de Luminy, Case 934, 13288 Marseille Cedex 9, France;2. URGI, INRA, Université Paris-Saclay, 78026 Versailles, France |
| |
| Abstract: | Enterodiol (END) possesses the estrogenic and antiestrogenic activities, which could prevent the development of breast cancer and prostate cancer, as well as menopausal syndrome.Previous studies in our laboratory set up a bio-transformation method for largely yielding secoisolariciresinol (SECO) from the substrate of defatted flaxseeds by strain Bacteroides uniformis ZL-I. In this research, another two single colonies, designated as strain ZL-II and strain ZL-III, were isolated, which were closely related to Eubacterium limosum species (ZL-II), and Eggerthella lenta species (ZL-III) on the basis of 16S rRNA gene sequence data. Under the combining actions of strains ZL-(I + II + III), END could be produced from defatted flaxseed directly, ZL-II was proved to possess the activities of demethylation, while ZL-III had the activities of dehydroxylation. Secoisolariciresinol diglucoside (SDG) existed in the form of oligomeric with 3-hydroxy-3-methyl glutaric acid in flaxseed could be efficiently transformed into END under the co-culture of strains ZL-(I + II + III), with the conversion rate of more than 90%. The method for mass-producing END from defatted flaxseed reported here is meaningful not only for the medicinal values of END but also for the resource utilization of flaxseed materials. |
| |
| Keywords: | Enterodiol Defatted flaxseed Biotransformation |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
