CTL induction by DNA vaccine with Toxoplasma gondii-HSP70 gene |
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| Institution: | 1. Pediatric Infectious Disease Unit, Soroka University Medical Center, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel;2. Parasitology Laboratory, Soroka University Medical Center, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva, Israel;1. Laboratory of Helminthology, Institute of Veterinary Medicine, Zaisan, Ulaanbaatar, Mongolia;2. Department of Parasitology, Asahikawa Medical University, Asahikawa, Japan;3. Laboratory of Protozoology, Institute of Veterinary Medicine, Zaisan, Ulaanbaatar, Mongolia;4. Department of Medical Biology and Histology, School of Biomedicine, Health Science University of Mongolia, Ulaanbaatar, Mongolia;5. Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX, USA;1. Internal Medicine Department, Groupement Hospitalier Sud, Hospices Civils de Lyon, France;2. Thoracic Surgery Department, Groupement Hospitalier Sud, Hospices Civils de Lyon, France;3. Visceral Surgery Department, Groupement Hospitalier Sud, Hospices Civils de Lyon, France;4. Laboratory of Parasitology, Groupement Hospitalier Nord, Hospices Civils de Lyon, France;5. Université Claude Bernard, Lyon 1, France |
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| Abstract: | Toxoplasma gondii-derived heat shock protein 70 (T.g.HSP70) is a tachyzoite-specific virulent molecule. The DNA vaccine with T.g.HSP70 gene targeting peripheral epidermal or dermal dendritic cells (DC) induces in vivo DC maturation and successive early Th1 polarization at the draining lymph nodes (dLN) of C57BL/6 mice. In the present study, induction of cytotoxic T lymphocytes (CTL) has been explored. The CTL specific for a syngeneic DC line, DC2.4, either transfected with T. g.HSP70 gene or pulsed with recombinant T. g.HSP70 are induced in the spleen of the vaccinated mice. This CTL lyses T. gondii-infected, but not uninfected, DC2.4. Both CD8+ and CD4+ CTL are induced by the vaccine, and Fas/Fas ligand-mediated cytolysis dominantly participates in their CTL activities. Adoptive transfer experiments reveal that the vaccine-induced CD8+ or CD4+ T cells possess a protective role for toxoplasmosis at both acute and chronic phases of infection. |
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