Enhanced acetone/butanol/ethanol production by Clostridium beijerinckii IB4 using pH control strategy |
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| Institution: | 1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Puzhu South Road 30#, Nanjing 211816, People''s Republic of China;2. Institute of Chemical Industry of Forest Products (CAF), Nanjing 210042, People''s Republic of China;1. Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia;2. School of Biosciences and Biotechnology, Faculty of Sciences and Technology, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia;3. School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia;1. LEQUIA, Institute of the Environment, University of Girona, Campus Montilivi, E-17071 Girona, Catalonia, Spain;2. Group of Molecular Microbial Ecology, Institute of Aquatic Ecology (IEA), University of Girona, Campus Montilivi, E-17071 Girona, Catalonia, Spain;1. Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125 Napoli, Italy;2. Department of Life Sciences and Technology / Bioprocess Engineering, Beuth University of Applied Sciences Berlin, Seestrasse 64, 13347 Berlin, Germany;1. College of Forestry, Northwest A&F University, 3 Taicheng Road, 712100 Yangling, China;2. School of Forest Sciences, University of Eastern Finland, P.O. Box 111, FI80101 Joensuu, Finland;3. School of Pharmacy, University of Eastern Finland, P.O. Box 1627, FI70211 Kuopio, Finland |
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| Abstract: | PH is an essential factor for acetone/butanol/ethanol (ABE) production using Clostridium spp. In this study, batch fermentations by Clostridium beijerinckii IB4 at various pH values ranging from 4.9 to 6.0 were examined. At pH 5.5, the ABE production was dominant and maximum ABE concentration of 24.6 g/L (15.7 g/L of butanol, 8.63 g/L of acetone and 0.32 g/L of ethanol) was obtained with the consumption of 60 g/L of glucose within 36 h. However, in the control (without pH control), an ABE concentration of 14.1 g/L (11.0 g/L of butanol, 3.01 g/L of acetone and 0.16 g/L of ethanol) was achieved with the consumption of 41 g/L of glucose within 40 h. A considerable improvement in the productivity of up to 93.8% was recorded at controlled pH in comparison to the process without pH control. To better understand the influence of pH on butanol production, the reducing power capability and NADH-dependent butanol dehydrogenase activity were investigated, both of which were significantly improved at pH 5.5. Thus, the pH control technique is a convenient and efficient method for high-intensity ABE production. |
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| Keywords: | Butanol pH control Reducing power Butanol dehydrogenase |
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