Optimization of ultrasound-assisted extraction conditions for total phenols with anti-hyperglycemic activity from Psidium guajava leaves |
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| Affiliation: | 1. Department of Animal Science and Biotechnology, Tunghai University, 1727, Section 4, Taiwan Boulevard, Taichung 40704, Taiwan, ROC;2. Department of Food Science, Tunghai University, 1727, Section 4, Taiwan Boulevard, Taichung 40704, Taiwan, ROC;3. Best Center for Cellular Nutrition, Han-Sient Trading Co., Ltd., 3F., 57, Section 2, Zhonghua Road, Sinzhuang District, New Taipei City 24246, Taiwan, ROC;1. Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo 25280, Coahuila, Mexico;2. Department of Biotechnology, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, D.F., Mexico;3. Animal Nutrition Department, Universidad Autónoma Agraria Antonio Narro, Saltillo 25315, Coahuila, Mexico;1. Istanbul Technical University, Faculty of Chemical and Metallurgical Engineering, Department of Food Engineering, 34469 Maslak, Istanbul, Turkey;2. TUBITAK, Marmara Research Center Food Institute, 41470 Kocaeli, Turkey;1. Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Avenida Vasco de Quiroga 4871, 05348 México D.F., México;2. Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, 09340 México D.F.,México;1. Department of Animal Science and Biotechnology, Tunghai University, 1727, Section 4, Taiwan Boulevard, Taichung 40704, Taiwan, ROC;2. Department of Food Science, Tunghai University, 1727, Section 4, Taiwan Boulevard, Taichung 40704, Taiwan, ROC;3. Best Center for Cellular Nutrition, Han-Sient Trading Co., Ltd., 13F., 665, Bannan Road, Zhonghe Dist., New Taipei City 23557, Taiwan, ROC |
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| Abstract: | The high concentration of total phenolic compounds (TPC) in Psidium guajava leaf extracts (GvEx) is correlated to its anti-hyperglycemic activity. In this study, we established the optimum ultrasound extraction conditions for maximizing TPC yield. The response surface methodology (RSM) was employed for empirical model building. The maximum value of TPC (26.12%) was obtained at solvent to solid ratio (v/w) of 12.1, extraction temperature of 59.8 °C, and extraction time of 5.1 min. The anti-hyperglycemic activity of GvEx was compared to the commonly used diabetic drug acarbose. The IC50 of GvEx for α-amylase and α-glucosidase inhibition was 50.5 μg/mL and 34.6 μg/mL, respectively. However, the IC50 of acarbose for α-amylase and α-glucosidase inhibition was 95.3 μg/mL and 1075.2 μg/mL, respectively. In conclusion, GvEx obtained under optimum extraction conditions had higher anti-hyperglycemic activity than acarbose. In addition, the recommended extraction procedures for GvEx save time and are environmentally friendly. |
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| Keywords: | Anti-hyperglycemic activity Response surface methodology Ultrasound |
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