Continuous production of ellagic acid in a packed-bed reactor |
| |
| Affiliation: | 1. Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Saltillo 25280, Coahuila, Mexico;2. Department of Biotechnology, Universidad Autónoma Metropolitana Iztapalapa, 09340 México, D.F., Mexico;3. Animal Nutrition Department, Universidad Autónoma Agraria Antonio Narro, Saltillo 25315, Coahuila, Mexico;1. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, PR China;2. Molecular Inflammation Research Center for Aging Intervention (MRCA), College of Pharmacy, Pusan National University, Busan 609-735, South Korea;3. Zhejiang Provincial Key Laboratory of Applied Enzymology, Yangtze Delta Region Institute of Tsinghua University, Jiaxing 314006, PR China;4. Department of Dermatology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul 135-710, South Korea;1. Istanbul Technical University, Faculty of Chemical and Metallurgical Engineering, Department of Food Engineering, 34469 Maslak, Istanbul, Turkey;2. TUBITAK, Marmara Research Center Food Institute, 41470 Kocaeli, Turkey;1. Department of Animal Science and Biotechnology, Tunghai University, 1727, Section 4, Taiwan Boulevard, Taichung 40704, Taiwan, ROC;2. Department of Food Science, Tunghai University, 1727, Section 4, Taiwan Boulevard, Taichung 40704, Taiwan, ROC;3. Best Center for Cellular Nutrition, Han-Sient Trading Co., Ltd., 3F., 57, Section 2, Zhonghua Road, Sinzhuang District, New Taipei City 24246, Taiwan, ROC;1. School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, Singapore;2. State Key Laboratory of Microbial Metabolism, and Laboratory of Molecular Biochemical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;1. Cosmax Bio INC, Jecheon 390-250, Republic of Korea;2. Department of Food and Nutrition, Korea University, Seoul 136-703, Republic of Korea;3. Department of Public Health Science, Graduate School, Korea University, Seoul 136-7033, Republic of Korea |
| |
| Abstract: | Ellagic acid is a high-value bioactive compound that is used in the food, cosmetic and pharmaceutical industries. The aim of this work was to develop a continuous system for ellagic acid production. Ellagitannase produced by solid-state fermentation and attached to polyurethane foam particles was used as a biocatalyst in a continuous bioreactor for the hydrolysis of ellagitannins from pomegranate by-product. A packed-bed reactor containing the biocatalyst (22.22 Units per gram of dry solid, U gds−1) was fed with a pomegranate ellagitannins solution (0.1%, w/v) at a flow rate of 0.27 mL min−1 at 60 °C. The bioreactor completed several biotransformations while maintaining the hydrolysis rate (60%) with a half-life of 10 continuous cycles of ellagic acid production. Volumetric productivity and ellagic acid yield were 1.09 g L−1 h−1 and 235.89 mg g−1 of pomegranate ellagitannins during the first 70 min of hydrolysis, respectively. The developed biocatalyst showed good operational and mechanical stability and may be successfully used for ellagitannin hydrolysis in a continuous system. This is the first report of high-yield continuous production of ellagic acid using an auto-immobilized enzyme. |
| |
| Keywords: | Ellagic acid Ellagitannase Biocatalyst Solid-state fermentation Continuous system |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
