Biochemical characterization of xylanase produced from Streptomyces sp. CS624 using an agro residue substrate |
| |
| Affiliation: | 1. Department of Pharmacy, Chosun University, Gwangju 501-759, Republic of Korea;2. Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 534-729, Republic of Korea;1. Posgrado en Producción Animal, Departamento de Zootecnia, Universidad Autónoma Chapingo. km 38.5, Carretera México-Texcoco, Chapingo, CP 56230, México;2. Departamento de Ciencias Básicas, Posgrado en Ciencias Biológicas, Universidad Autónoma de Aguascalientes. Av. Universidad 940, Cd. Universitaria, Aguascalientes, CP 20131, México;3. Posgrado en Biotecnología Agropecuaria, División de Estudios Posgrado e Investigación, Instituto Tecnológico El Llano Aguascalientes. Km 18.5 Carretera Aguascalientes-S.L.P., El Llano, Aguascalientes. CP 20330, México;1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi, China;2. College of Life Science and Technology, Guangxi University, Nanning 530004, Guangxi, China;3. Hainan Yangpu Vocational High School, Yangpu 578101, Hainan, China;1. Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing Forestry University, Nanjing 210037, People’s Republic of China;2. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People’s Republic of China;3. Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People’s Republic of China |
| |
| Abstract: | An extracellular and cellulase-free xylanase (EX624) was produced by Streptomyces sp. CS624 using an agricultural residue (wheat bran) as a growth substrate. EX624 was purified from culture supernatant using ammonium sulfate precipitation, ion exchange and gel filtration chromatography. The SDS-PAGE and the zymogram analysis of the purified xylanase indicated molecular mass of 40 kDa. Biochemical characterization of the purified EX624 revealed its highest activity at a temperature of 60 °C and pH 6.0. The xylanase was adequately stable in the pH range 4.5–10.0 and at temperatures ≤50 °C. EX624 displayed enhanced activity in the presence of several metal ions including Fe2+, Co2+ and Ca2+. HPLC results showed that EX624 was not only able to hydrolyze commercially available pure beechwood xylan to xylose, xylobiose and xylotriose, but also abundantly available lignocellulosic agricultural residues in nature such as wheat bran to xylooligosaccharides. |
| |
| Keywords: | Xylanase Agro residues Xylooligosaccharide |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
