Production of optically pure d-lactate from CO2 by blocking the PHB and acetate pathways and expressing d-lactate dehydrogenase in cyanobacterium Synechocystis sp. PCC 6803 |
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| Affiliation: | 1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;2. Department of Cellular Biology, University of Science and Technology of China, Hefei, China;1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211800, PR China;2. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing 211800, PR China;1. Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, USA;2. Biological and Materials Science Center, Sandia National Laboratories, Livermore, CA 94550, USA;1. College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan;2. Japan Science and Technology Agency (JST), Precursory Research for Embryonic Science and Technology (PRESTO), 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan;3. RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Yokohama 230-0045, Japan;4. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Kobe 657-8501, Japan;1. Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, United States;2. Microbiology Doctoral Training Program, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, United States;3. Department of Environmental Chemistry and Technology, University of Wisconsin-Madison, 660 N Park St, Madison, WI 53706, United States;4. Departmentof Chemistry and Biochemistry, University of Colorado-Boulder, 596 UCB, Boulder, CO 80309, United States;5. Renewable and Sustainable Energy Institute, University of Colorado-Boulder, 027 UCB, Boulder, CO 80309, United States |
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| Abstract: | Lactate is an important industrial material with numerous potential applications, and its production from carbon dioxide is very attractive. d-Lactate is an essential monomer for production of thermostable polylactide. The photoautotrophic prokaryote cyanobacterium Synechocystis sp. PCC 6803 represents a promising host for biosynthesis of d-lactate from CO2 as it only contains d-lactate dehydrogenase. The production of d-lactate from CO2 by an engineered strain of Synechocystis sp. PCC 6803 with overexpressing d-lactate dehydrogenase and a soluble transhydrogenase has been reported recently. Here, we report an alternative engineering strategy to produce d-lactate from CO2. This strategy involves blocking two competitive pathways, the native poly-3-hydroxybutyrate and acetate pathways from the acetyl-CoA node, and introducing a more efficient d-lactate dehydrogenase into Synechocystis sp. PCC 6803. The engineered strain of Synechocystis sp. PCC 6803 was capable of producing 1.06 g/L of d-lactate from CO2. This alternative strategy for the production of optically pure d-lactate could also be used to produce other acetyl-CoA-derived chemicals from CO2 by using engineered cyanobacteria. |
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| Keywords: | Cyanobacteria Rerouting of carbon flux Biosynthesis Carbon dioxide |
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