Optimization of the aeration and agitation speed of Aeribacillus palidus 418 exopolysaccharide production and the emulsifying properties of the product |
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| Institution: | 1. Dept of Mathematical and Informatics Sciences, Physical Sciences and Earth Sciences of Messina University, Viale Ferdinando Stagno D'' Alcontres 31, 98166 Messina, Italy;2. Le Studium Loire Valley Institute for Advanced Studies, 1 rue Dupanloup 45000, Orléans, France;3. Centre de Biophysique Moleculaire (CBM)-CNRS UPR 4301 du CNRS, rue Charles Sadron, 45071 Orleans CEDEX 2 France; Laboratoire Interfaces, Confinement, Matériaux et Nanostructures (ICMN) - UMR 7374 CNRS - Université d''Orléans, 1b rue de la Férollerie, CS 40059, 45071 Orléans cedex 2, France;4. Istituto Nazionale di Alta Matematica “F. Severi” – INDAM – Gruppo Nazionale per la Fisica Matematica – GNFMV, Piazzale Aldo Moro, 5, 00185 Roma, Italy;5. Dept. of Chemical, Biological, Pharmaceutical and Environmental Sciences, Messina University, Viale Ferdinando Stagno D'' Alcontres 31, 98166 Messina, Italy;6. Dept. of Earth and Marine Sciences, University of Palermo, Via Archirafi, 22, 90123 Palermo, Italy;1. Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal;2. UCIBIO – Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal;3. CICECO - Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal;4. LEAF - Linking Landscape, Environment, Agriculture and Food, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, 1349-017 Lisboa, Portugal;5. Pacific Biotech BP 140 289 Arue Tahiti 98 701, French Polynesia, France;6. AiMB (Advices in Marine Biotechnology), 17 Rue d''Ouessant, 29280 Plouzané, France;7. LAQV-REQUIMTE - Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal |
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| Abstract: | The specific properties of exopolysaccharides (EPS) from thermophilic microorganisms have attracted interest in their optimized production. In this study, the ability of Aeribacillus pallidus 418 to grow and produce polysaccharide in a 5-l stirred tank bioreactor was investigated. Agitation rates of 100, 200, 600, 900, and 1100 revolutions per minute (rpm), at an air flow rate of 0.5 gas volumes per unit medium volume per minute (vvm), and aeration rates of 0.25, 0.5, 1.0, and 1.5 vvm, at an agitation rate of 900 rpm, were examined. A maximum EPS yield of 170 μg/ml has been registered in a single impeller bioreactor equipped with an original Narcissus impeller at agitation speed of 900 rpm, with an aeration rate of 0.5 vvm. The bioprocess oxygen uptake rate (OUR) and oxygen mass transfer coefficient (KLa) were evaluated. The emulsifying properties of the specific EPS produced by A. pallidus 418 were determined. Stable oil-in-water emulsions, a low level of separated water phase and high dispersion stability were found, which together demonstrate the prospects for the industrial exploration of EPS production. Enhanced synergism between the A. pallidus 418 synthesized EPS and various commercially used hydrocolloids was observed; superior synergy was achieved in combination with xanthan gum. |
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| Keywords: | Exopolysaccharide Thermophilic fermentation Agitation and aeration Emulsifying properties |
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