Electrically conductive magnetite particles enhance the kinetics and steer the composition of anaerobic TCE-dechlorinating cultures |
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| Institution: | 1. Water Research Institute (IRSA), National Research Council (CNR), Via Salaria km 29,300, 00015 Monterotondo, Italy;2. Department of Chemistry, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy;1. College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, PR China;2. The Belt and Road Information Research Institute, Hangzhou Dianzi University, Hangzhou 310018, PR China;3. Zhejiang Province Environmental Engineering Co. Ltd, Hangzhou 310012, PR China;4. M-U-T Maschinen-Umwelttechnik-Transportanlagen GmbH, Stockerau 2000, Austria;1. Water Research Institute (IRSA), National Research Council (CNR), 00015, Monterotondo, RM, Italy;2. Department of Earth and Environmental Sciences, University of Milano-Bicocca, 20126, Milan, Italy;3. Department of Chemistry, Sapienza University of Rome, 00185, Rome, Italy;4. School of Environmental Engineering, Technical University of Crete, 73100, Chania, Greece;1. Technische Universität Berlin, Water Quality Control, Str. des 17. Juni 135, 10623 Berlin, Germany;2. Technische Universität Berlin, Mineralogy, Ackerstr. 76, 13355 Berlin, Germany;3. Technische Universität Berlin, Center for Electron Microscopy (ZELMI), Str. des 17. Juni 135, 10623 Berlin, Germany;1. College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China;2. Key Laboratory of Marine Environmental and Ecology, Ministry of Education, Ocean University of China, Qingdao, 266100, China;3. Biosystems Engineering Department, Auburn University, Auburn, AL, 36849-5417, United States;1. Water Research Institute (IRSA), National Research Council (CNR), Italy;2. Department of Chemistry, Sapienza University of Rome, Italy;3. Institute for the Study of Nanostructured Materials (ISMN), National Research Council (CNR), Italy;4. School of Civil Engineering and Geosciences, Newcastle University, United Kingdom;5. Center for Microbial Ecology and Technology (CMET), Ghent University, Belgium |
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| Abstract: | The interaction of anaerobic dechlorinating cultures with soil and aquifer geochemical components is largely unknown, although this has potentially a major impact on the bioremediation of chlorinated solvent-contaminated sites. In this study, we found that addition of magnetite (Fe3O4) – the end-product of Fe(III)-reduction by dissimilatory iron reducing bacteria – to anaerobic dechlorinating cultures enhances the kinetics of trichloroethene dechlorination up to 1.5-times, compared to unamended controls. Specifically, a low concentration (approx. 10 mg/L as total Fe) of small size particles (200 nm-filtered) resulted in a greater stimulatory effect compared to the addition of a higher concentration (approx. 300 mg/L as total Fe) of unfiltered particles. Notably, Desulforomonas spp. were substantially enriched in microcosms supplemented with magnetite, whereas Dehalococcoides mccartyi spp. was found to be markedly inhibited or outcompeted. Multiple lines of evidence, including the direct visualization of microbial cells and magnetite particles via Confocal Laser Scanning Microscopy (CLSM), suggest that electrically conductive particles promoted the establishment of a cooperative metabolism, based on direct interspecies electron transfer, between dechlorinating and non-dechlorinating microorganisms. |
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| Keywords: | Direct interspecies electron transfer (DIET) Magnetite nanoparticles Reductive dechlorination TCE |
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