High-loading oil palm empty fruit bunch saccharification using cellulases from Trichoderma koningii MF6 |
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| Affiliation: | 1. Institute of Biochemical Engineering, Technische Universität München, Boltzmannstr. 15, 85748 Garching, Germany;2. Department of Chemistry, Technische Universität München, Lichtenbergstr. 4, 85748 Garching, Germany;1. State Key Laboratory of Applied Organic Chemistry, Institute of Biochemical Engineering & Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China;2. The First Affiliated Hospital of Lanzhou University, Lanzhou University, Lanzhou 730000, China;1. State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China;2. Provincial Key Laboratory of Green Processing Technology and Product Safety of Natural Products, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China;3. Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China;4. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China;1. Departamento de Genética, Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, Campinas-SP, Brazil;2. Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA;1. Frontier Research Labs., Institute for Innovation, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-8681, Japan;2. Graduate School of Materials Science, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan |
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| Abstract: | Strain Trichoderma koningii D-64 was improved for enhanced cellulase production. A potential mutant MF6 was obtained and its enzymes contained filter paper cellulase (FPase), carboxymethylcellulase (CMCase), β-glucosidase and xylanase with respective activities of 2.0, 1.3, 2.0 and 3.0 folds of those for the parental strain. MF6 cellulases showed enhanced hydrolysis performance for the treated lignocellulosic biomass. Hydrolysis of treated oil palm empty fruit bunch (OPEFB), horticulture wastes (HW) and wood chips (WC) resulted in cellulose to glucose conversion of 96.3 ± 2.2%, 98.2 ± 3.0% and 81.9 ± 1.4%, respectively. The corresponding conversions of xylan to xylose were 96.9 ± 1.5%, 95.0 ± 2.2% and 76.1 ± 3.1%. Consistently, high sugar yield of 770–844 mg/g biomass was obtained for high-loading (10–16%, w/v) of OPEFB hydrolysis and sugar titer of 135.1 g/L was obtained for 16% (w/v) OPEFB loading at 96 h. In addition, MF6 enzymes alone performed equally well for high-loading OPEFB hydrolysis compared to the enzyme mixture of β-glucosidase from Aspergillus niger and cellulase from T. reesei Rut C30. |
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| Keywords: | Cellulose Biomass hydrolysis Oil palm empty fruit bunch High biomass loading |
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