Reversible immobilization of glucoamylase onto magnetic polystyrene beads with multifunctional groups |
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| Institution: | 1. State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Institute of Biochemical Engineering & Environmental Technology, Lanzhou University, Lanzhou 730000, China;2. The First Affiliated Hospital of Lanzhou University, Lanzhou University, Lanzhou 730000, China;3. School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China;1. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe 657-8501, Japan;2. Organization of Advanced Science and Technology, Kobe University, Kobe 657-8501, Japan;3. Department of Applied Chemistry, Graduate School of Engineering and Center for Future Chemistry, Kyushu University, 744 Moto-oka, Fukuoka 819-0395, Japan;1. Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China;2. Key Laboratory of Designed Synthesis and Application of Polymer Material, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, China;3. Instrumental Analysis and Research Centre, Sun Yat-Sen University, Guangzhou 510275, China;1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic;2. Masaryk Memorial Cancer Institute, Žlutý kopec 7, 656 53 Brno, Czech Republic;1. Institute for Solar Energy Systems, School of Physics and Engineering, Sun Yat-Sen University, Guangzhou 510006, PR China;2. Department of Applied Chemistry, the College of Science, South China Agricultural University, Guangzhou 510640, PR China;1. School of Materials Science and Engineering, Qilu University of Technology, Key Laboratory of Amorphous and Polycrystalline Materials, Key Laboratory of Processing and Testing Technology of Glass Functional Ceramics of Shandong Province, Jinan 250353, PR China;2. State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, PR China |
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| Abstract: | A novel and simple process for the surface functionalization of micron-sized monodisperse magnetic polystyrene (PS) microbeads was reported. The polystyrene seed particles were prepared prior to the dispersion polymerization method. Afterwards, series of surface chemical modifications on polystyrene microspheres were conducted, and three end-functional microspheres with carboxyl, imidazolyl and sulphydryl groups were obtained. The functional magnetic polystyrene microspheres were prepared by impregnation and subsequent precipitation of ferric and ferrous ions into the polystyrene particles. Finally, the functional magnetic polystyrene was used for the reversible immobilization of glucoamylase via metal-affinity adsorption. The results indicated that the obtained immobilized glucoamylase presented excellent reusability, applicability, magnetic response and regeneration of supports. The magnetic PS microspheres retained >65% of its initial activity at 65 °C over 6 h; and the lowest residual activity of immobilized glucoamylase prepared by regenerated supports still remained about 50% of the initial activity after the 10th cycles. |
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| Keywords: | Composite materials Magnetic materials Polymers Microstructure Electron microscopy |
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