Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cytometry |
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| Institution: | 1. State Key Laboratory of Pulp and Paper Engineering, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China;2. Provincial Key Laboratory of Green Processing Technology and Product Safety of Natural Products, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, PR China;3. Key Laboratory of Renewable Energy, Chinese Academy of Sciences, Guangzhou 510640, PR China;4. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China;1. Megazyme International Ireland, Bray Business Park, Southern Cross Road, Bray, County Wicklow, Ireland;2. Centre de Recherches sur les Macromolécules Végétales (CERMAV, UPR-CNRS 5301), Affiliated with Grenoble Alpes University, Member of Institut de Chimie Moléculaire de Grenoble (ICMG, FR-CNRS 2607) and Institut Carnot PolyNat, BP53, 38041 Grenoble Cedex 9, France;1. Departamento de Reprodução Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, São Paulo, Brazil;2. Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, São Paulo, Brazil;3. Unidade de Pesquisa e Desenvolvimento de Registro-Pólo Regional do D.S.A. do Vale do Ribeira/APTA, Registro, São Paulo, Brazil;4. Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India;1. CAS Key Laboratory of Renewable Energy, Guangzhou 510640, PR China;2. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, PR China;3. Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, PR China;4. University of Chinese Academy of Sciences, Beijing 100049, PR China;1. Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, Piazzale Europa 1, 34127 Trieste, Italy;2. Novozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark |
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| Abstract: | The effect of ten typical organic acids and five aldehydes present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans was evaluated by flow cytometry. Overall, organic acids affected the cell membrane integrity of T. fermentans more significantly than that of aldehydes albeit aldehydes are more toxic to T. fermentans. The PI (Propidium Iodide) uptake rate of T. fermentans’ cells gradually decreased as fermentation going on, indicating that T. fermentans could overcome the inhibition of organic acids or aldehydes by adaption. Interestingly, in some cases, the effect of organic acids or aldehydes on the cell membrane integrity of T. fermentans was well related to their hydrophobicity. However, for the outliers, no obvious similar phenomena were observed. Thus, the attack on hydrophobic sites of cell membrane was not the only determinant for the damage of organic acids or aldehydes on cell membrane integrity of T. fermentans. |
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| Keywords: | Organic acid Aldehyde Cell membrane integrity Flow cytometry Lignocellulosic hydrolysate |
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