Concurrent purification and antioxidant activity of phycobiliproteins from Lyngbya sp. A09DM: An antioxidant and anti-aging potential of phycoerythrin in Caenorhabditis elegans |
| |
Institution: | 1. BRD School of Biosciences, Vadtal Road, Satellite Campus, Post Box No 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Gujarat, India;2. Department of Biotechnology, Shree A.N. Patel PG Institute, Sardar Patel University, Anand 388 001 Gujarat, India;3. The Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USA;1. Mer Molécule Santé, EA 2160, Université de Nantes, Pôle Mer et Littoral, 2 rue de la Houssinière, BP 92208, 44322 Nantes Cedex 3, France;2. Laboratoire de Génie des Procédés Environnement Agro-alimentaire (GEPEA), UMR CNRS 6144, Université de Nantes, Pôle Mer et Littoral, 37 Boulevard de l’Université, BP 406, 44602 Saint-Nazaire Cedex, France;1. Department of Botany, Ayya Nadar Janaki Ammal College, Sivakasi, Tamilnadu, India;2. Centre for Advanced Studies in Botany, University of Madras, Guindy, Chennai, Tamilnadu, India;3. Department of Virology, King Institute of Preventive Medicine and Research, Guindy, Chennai, Tamilnadu, India;4. Department of Botany, Annamalai University, Annamalai Nagar, Chidambaram, Tamilnadu, India;1. Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;2. College of Energy, Xiamen University, Xiamen 361005, China;3. The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen 361005, China |
| |
Abstract: | The present study probes into the purification of phycobiliproteins, and characterization of their in vitro anti-oxidant activity. Moreover, the study also demonstrates the use of antioxidant virtue of phycoerythrin in moderating the phenomenon of aging in Caenorhabditis elegans. Phycoerythrin, phycocyanin and allophycocyanin were purified successfully from Lyngbya sp. A09DM by ammonium sulfate fractionation appended with Triton X-100 intercession. The success of protocol was examined by a series of biochemical characterization like SDS-PAGE, native-PAGE, UV–visible spectroscopy and fluorescence spectroscopy ensuring purity, integrity and functionality of purified phycoerythrin, phycocyanin and allophycocyanin. Purified phycobiliproteins were evaluated for antioxidant and metal ion chelating activity by various in vitro antioxidant assay systems. Results showed significant and dose-dependent antioxidant as well as metal chelating potential of all phycobiliproteins in decreasing order of phycoerythrin > phycocyanin > allophycocyanin. Expansion in lifespan and improvement in pharyngeal pumping of C. elegans were noticed upon pre-treatment with phycoerythrin (100 μg ml?1). Moreover, phycoerythrin mediated increase in worm survival under oxidative stress revealed that the life expansion effect of phycoerythrin on nematode is in part by an action of its antioxidant virtue. These results collectively added up evidence in favor of the ‘free-radical theory of aging’. The present report, for the first time, describes antioxidant potential of phycoerythrin and its use in extending life-span of C. elegans. |
| |
Keywords: | Phycobiliproteins Triton X-100 Anti-aging activity |
本文献已被 ScienceDirect 等数据库收录! |
|