Production of polyhydroxyalkanoates using hydrolysate of spent coffee grounds |
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| Institution: | 1. Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic;2. Institute of Chemistry and Technology of Environmental Protection, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic;3. Institute of Material Chemistry, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic;1. Institute of Biophysics SB RAS, Akademgorodok 50, 660036 Krasnoyarsk, Russian Federation;2. Siberian Federal University, 79 Svobodny pr., 660041 Krasnoyarsk, Russian Federation;1. IBB – Institute for Biotechnology and Bioengineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal;2. Biotrend – Inovação e Engenharia em Biotecnologia, S.A., Biocant Park, Núcleo 04 – Lote 2, 3060-197 Cantanhede, Portugal;3. CIIEM – Centro de Investigação Interdisciplinar Egas Moniz, ISCSEM, Campus Universitário, Quinta da Granja, 2829-511 Monte de Caparica, Portugal;1. Universidade Federal do Ceará, Departamento de Engenharia Química, Fortaleza, CE, Brazil;2. Universidade Federal Rural do Semiárido, Departamento de Agrotecnologia e Ciências Sociais, Mossoró, RN, Brazil;1. Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic;2. Department of Food Science and Technology, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic;1. Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal;2. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands |
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| Abstract: | Spent coffee grounds (SCG) are solid fraction wastes deriving from coffee industries, the disposal of which represents a serious environmental issue. This work aims at the conversion of hydrolysate of SCG (SCGH) into polyhydroxyalkanoates (PHA) by Burkholderia cepacia. The bacteria was capable of SCGH utilization and production of copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate P(HB-co-HV]. Levulinic acid present in SCGH probably served as the precursor of 3HV for the copolymer biosynthesis. To improve the PHA yields, various detoxification methods were tested. The extraction of polyphenols from SCG by ethanol prior to the hydrolysis seems to be the most promising, since, apart from the fact that it enhanced the PHA yields by about 25%, polyphenols extracted from SCG may represent important side products, because they might be used for the production of functional foods and other high value products. |
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| Keywords: | Spent coffee grounds Polyhydroxyalkanoates PHA Detoxification |
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