Improving the lactic acid production of Actinobacillus succinogenes by using a novel fermentation and separation integration system |
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| Affiliation: | 1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China;2. Graduate University of Chinese Academy of Sciences, Beijing 100049, PR China;3. College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, PR China;1. DSM Biotechnology Center, Alexander Fleminglaan 1, 2613 AX Delft, The Netherlands;2. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands;1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu 211800, China;2. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, Jiangsu 211800, China;3. Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, 4001 Discovery Dr. Suite N321, Boulder 80309, USA;1. Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1421, C.P. 44430 Guadalajara, Jalisco, Mexico;2. Unidad de Tecnología Agroalimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ), A.C. Av. Normalistas 800, Colinas de la Normal, C.P. 44270 Guadalajara, Jalisco, Mexico;3. Departamento de Madera Celulosa y Papel, Universidad de Guadalajara, Km. 15.5 carretera Guadalajara-Nogales, C.P. 45110 Zapopan, Jalisco, Mexico;1. School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University avenue, Muang district, Nakhon Ratchasima 30000, Thailand;2. Cassava Research Center, Suranaree University of Technology, 111 University avenue, Muang district, Nakhon Ratchasima 30000, Thailand;3. Department of Chemical and Biomolecular Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan District, Rayong 21210, Thailand;1. West Pomeranian University of Technology, Faculty of Chemical Technology and Engineering, Al.Piastow 42, 70-065 Szczecin, Poland;2. Maritime University of Szczecin, Faculty of Economics and Transport Engineering, ul.H.Poboznego 11, 70-507 Szczecin, Poland |
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| Abstract: | This work describes the development of a novel integrated system for lactic acid production by Actinobacillus succinogenes. Fermentation and separation were integrated with the use of a microfiltration (MF) membrane, and lactic acid was recovered by resin adsorption following MF. The fermentation broth containing residual sugar and nutrients was then recycled back into the fermenter after lactic acid adsorption. This novel approach overcame the problem of product inhibition and extended the cell growth period from 41 h to 120 h. Production of lactic acid was improved by 23% to 183.4 g L−1. The overall yield and productivity for glucose were 0.97 g g−1 and 1.53 g L−1 h−1, respectively. These experimental results indicate that the integrated system could benefit continuous production of lactic acid at high levels. |
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| Keywords: | Lactic acid Membrane filtration Adsorptive separation Process integration |
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