Separation,purification, anticoagulant activity and preliminary structural characterization of two sulfated polysaccharides from sea cucumber Acaudina molpadioidea and Holothuria nobilis |
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| Affiliation: | 1. Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China;2. School of Biology and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212018, PR China;1. Borneo Marine Research Institute, University Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia;2. Biotechnology Research Institute, University Malaysia Sabah, 88400 Kota Kinabalu, Sabah, Malaysia;1. N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prospect 47, Moscow 119991, Russia;2. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Prospect 100 let Vladivostoku 159, Vladivostok 690022, Russia;3. EaStCHEM School of Chemistry, Joseph Black Building, The King''s Buildings, University of Edinburgh, Edinburgh EH9 3JJ, United Kingdom,;4. GlycoMar Ltd., European Centre for Marine Biotechnology, Dunstaffnage Marine Laboratory, Oban, Argyll PA37 1QA, United Kingdom,;5. Scottish Association for Marine Science, Scottish Marine Institute, Oban, Argyll PA37 1QA, United Kingdom,;6. Glycosciences Laboratory, Hammersmith Campus, Imperial College London, London W12 0NN, United Kingdom,;12. National Institute of Biological Standards and Controls, South Mimms, Potters Bar, Hertfordshire EN6 3QG, United Kingdom,;8. Complex Carbohydrate Research Center, the University of Georgia, Athens, Georgia 30602, and;9. School of Chemistry, National University of Ireland Galway, University Road, Galway, Ireland;1. Matís, Vínlandsleið 12, IS-113 Reykjavik, Iceland;2. Faculty of Pharmaceutical Sciences, University of Iceland, Hagi, Hofsvallagata 53, IS-107 Reykjavik, Iceland;3. Centre for Rheumatology Research and Department of Immunology, Landspitali-The National University Hospital of Iceland, IS-101 Reykjavik, Iceland;4. Faculty of Medicine, Biomedical Center, Vatnsmyrarvegur 16, IS-101 Reykjavik, Iceland;5. Department of Pharmacognosy, Institute of Pharmacy, University of Oslo, PO Box 1068, N-0316, Oslo, Norway;1. Laboratoire de Génétique, Biodiversité et Valorisation des Bioressources (LR11ES41), University of Monastir, Tunisia;2. Laboratoire d’Amélioration de Plantes et Valorisation des Agro-Ressources (LAPVA), National School of Engineering of Sfax, University of Sfax, Km. 4 Road Soukra, 3038 Sfax, Tunisia;3. Université Paris Diderot, INSERM U 1148, Paris, France;4. Laboratoire de Chimie Hétérocyclique, Produits Naturels et Réactivité (LR11ES39), Equipe: Chimie Médicinale et Produits Naturels, Faculté des Sciences de Monastir, Université de Monastir, 5000 Monastir, Tunisia;5. INSERM U1148, Laboratory for Vascular Translational Science (LVTS), Institut Galilée, Université Paris 13, Sorbonne Paris Cité, 99 Avenue Jean-Baptiste Clément, Villetaneuse F- 93430, France |
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| Abstract: | In this study, we isolated two fucosylated polysaccharide sulfates (ACP and HOP) from sea cucumber Acaudina molpadioidea and Holothuria nobilis, with an average molecular weight of 90.8 and 135.8 kDa, respectively. We investigated and compared their anticoagulant activities through anticoagulant assay. Our data showed that both polysaccharides possessed good anticoagulant activity, but HOP's activity was higher than that of ACP. Due to the different anticoagulant activities of ACP and HOP, we compared the preliminary structural characterizations of these two sulfated polysaccharides, and found that both ACP and HOP consisted of β-d-glucuronic acid, β-d-N-acetyl-galactosamine, α-l-fucose and sulfate groups. ACP and HOP had almost identical ratios of glucuronic acid, N-acetyl-galactosamine and fucose. However, the sulfate contents and sulfation patterns of fucose residues of ACP and HOP were obviously different. There were 4-O-sulfated fucose, 3,4-O-disulfated fucose and 2,4-O-disulfated fucose in ACP, but only 3-O-sulfated fucose and 2,4-O-disulfated fucose were present in HOP. Therefore, their distinct anticoagulant activities might be due to the different sulfate contents and sulfation patterns of their fucose residues. |
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| Keywords: | Sea cucumber Fucosylated polysaccharide sulfate Anticoagulant activity Structure |
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